Kipre B G, Guessennd N K, Koné M W, Gbonon V, Coulibaly J K, Dosso M
Institut Pasteur Côte d'Ivoire, Département de Bactériologie/Virologie, Centre National de Reference Antibiotiques, 3 Avenue de l'Université Cocody, B.P 490, Abidjan 01, Côte d'Ivoire.
Université Nangui Abrogoua Côte d'Ivoire, B.P 801, Abidjan 01, Côte d'Ivoire.
BMC Complement Altern Med. 2017 Mar 27;17(1):170. doi: 10.1186/s12906-017-1681-8.
Tieghemella heckelii (Sapotaceae) is a medicinal plant used in Africa, particularly in Côte d'Ivoire for treating various diseases including infections. Identification of prospective antibacterial compounds from stem bark of this plant as a result of its medicinal virtue, led to screening activity against methicillin resistant bacteria.
Six extracts (hexane, chloroform, ethyl acetate, ethanol, methanol and sterile distilled water) were prepared and tested on methicillin resistant Staphylococcus aureus (MRSA) using broth microdilution method for activity assessment. From this experiment, the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the plant extracts were determined in sterile 96-well microplates in order to search for both bacteriostatic and bactericidal effects. Afterwards, data analysis was performed using GraphPad Prism5 software (One-way ANOVA and Turkey Multiple Comparison test). The results were then presented as Mean ± SD for experiment repeated three times.
Four extracts (ethyl acetate, methanol, ethanol and sterile distilled water) showed credible potency, with strong, significant, and moderate growth inhibition of the MRSA tested. The MIC values which varied from 45 μg/mL to 97 μg/mL according to microbial phenotype, resolutely established the activity of the plant extracts. Additionally, the MBC values which varied, depending on the type of bacteria strain, revealed the bacteriostatic and bactericidal effects of the active extracts against Methicillin-resistant Staphylococcus aureus.
The present study is a confirmation of the therapeutic potential of Tieghemella heckelii and its promising contribution to the discovery of a novel antibacterial drug pertaining to these resistant strains.
黑氏铁列木（山榄科）是一种在非洲使用的药用植物，特别是在科特迪瓦用于治疗包括感染在内的各种疾病。由于这种植物的药用价值，对其茎皮中潜在的抗菌化合物进行鉴定，从而开展了针对耐甲氧西林细菌的筛选活动。
制备了六种提取物（己烷、氯仿、乙酸乙酯、乙醇、甲醇和无菌蒸馏水），并采用肉汤微量稀释法对耐甲氧西林金黄色葡萄球菌（MRSA）进行活性评估测试。通过该实验，在无菌96孔微孔板中测定植物提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），以寻找抑菌和杀菌效果。之后，使用GraphPad Prism5软件进行数据分析（单因素方差分析和土耳其多重比较检验）。实验重复三次，结果以平均值±标准差表示。
四种提取物（乙酸乙酯、甲醇、乙醇和无菌蒸馏水）显示出可靠的效力，对测试的MRSA有强烈、显著和中等程度的生长抑制作用。根据微生物表型，MIC值在45μg/mL至97μg/mL之间变化，明确证实了植物提取物的活性。此外，MBC值因细菌菌株类型而异，揭示了活性提取物对耐甲氧西林金黄色葡萄球菌的抑菌和杀菌作用。
本研究证实了黑氏铁列木的治疗潜力及其对发现针对这些耐药菌株的新型抗菌药物的潜在贡献。
