Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia.
Food Chem. 2012 Dec 1;135(3):1419-24. doi: 10.1016/j.foodchem.2012.05.120. Epub 2012 Jun 9.
The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of Limoniastrum guyonianum Boiss. (Zita) were investigated in this study. The ethyl acetate phase exhibited a significant antioxidant activity as judged by total antioxidant activity, DPPH test and reducing power. Fractionation of this extract by centrifugal partition chromatography (CPC) using quaternary Arizona solvent systems composed of n-heptane/ethyl acetate/methanol/water led to ten fractions. The antioxidant capacities of these fractions were assessed using the same previous tests. Fraction 8 showed the highest antioxidant capacity (1291.1mg GAE/g DR), the power ability to quench DPPH radical (IC(50)=2μg/ml) and to reduce Fe(3+) (EC(50)=65μg/ml). From this fraction, three powerful flavonoids were isolated (1-3): gallocatechin (1), epigallocatechin (2) and epigallocatechin-3-O-gallate (3). These findings suggest that the antioxidative property of L. guynianum is may be related to the presence of these flavonoids, which can be used in various industrial fields.
本研究考察了 Limoniastrum guyonianum Boiss.（Zita）粗提物、水相和乙酸乙酯相的抗氧化能力。乙酸乙酯相的总抗氧化活性、DPPH 试验和还原能力表明其具有显著的抗氧化活性。使用由正庚烷/乙酸乙酯/甲醇/水组成的四级 Arizona 溶剂系统通过离心分配色谱（CPC）对该提取物进行分级，得到了十个馏分。使用相同的先前试验评估了这些馏分的抗氧化能力。馏分 8 显示出最高的抗氧化能力（1291.1mg GAE/g DR），能够淬灭 DPPH 自由基（IC（50）=2μg/ml）和还原 Fe（3+）（EC（50）=65μg/ml）。从该馏分中分离出三种强效黄酮类化合物（1-3）：没食子儿茶素（1）、表没食子儿茶素（2）和表没食子儿茶素-3-O-没食子酸酯（3）。这些发现表明，L. guynianum 的抗氧化特性可能与这些黄酮类化合物的存在有关，这些黄酮类化合物可用于各个工业领域。
