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从土耳其深紫色茄子果皮中获得的不同富含酚类组分的抗氧化活性

Antioxidant Activity of Different Phenolic-Rich Fractions Obtained From Peels of Turkish Dark Purple Eggplant.

作者信息

Djouadi Assia, Yılmazer Hakan, Djouadi Anfal, Çakır Bilal

机构信息

Department of Metallurgical and Materials Engineering, Faculty of Chemical and Metallurgical Yıldız Technical University Istanbul Turkey.

Health Biotechnology Joint Research and Application Center of Excellence Esenler Istanbul Turkey.

出版信息

Food Sci Nutr. 2025 Jun 27;13(7):e70521. doi: 10.1002/fsn3.70521. eCollection 2025 Jul.

Abstract

This study investigated the extraction of phenolic compounds and the antioxidant potential of eggplant peel using various extraction methods and GRAS solvent systems. Ethanol absolute and (EtOH: water) (70:30) system solvents were used separately to extract phenolic compounds, while the (EtOH: water: citric acid) (70:30:0.5) system was utilized for anthocyanin extraction. Otherwise, to test the antioxidant activity of anthocyanin separately, an aqueous anthocyanin-rich fraction was recovered from eggplant peel crude extract using successive liquid/liquid extractions with solvents of different polarities. The presence of total phenolics, anthocyanins and proanthocyanidin were detected, and HPLC analysis was realized. The antioxidant activity was performed by the (DPPH) free radicals method. Soxhlet extraction using absolute ethanol provides a high yield of phenolic compounds. Otherwise, the anthocyanin extract showed a high level of anthocyanin contents. The liquid chromatographic analysis presented an important percentage of flavonoids. Soxhlet extract was the most effective in scavenging free radicals.

摘要

本研究采用多种提取方法和公认安全(GRAS)溶剂体系,对茄子皮中酚类化合物的提取及其抗氧化潜力进行了研究。分别使用无水乙醇和(乙醇:水)(70:30)体系溶剂提取酚类化合物,而(乙醇:水:柠檬酸)(70:30:0.5)体系用于提取花青素。此外,为单独测试花青素的抗氧化活性,采用不同极性的溶剂通过连续液/液萃取从茄子皮粗提物中回收富含花青素的水相部分。检测了总酚、花青素和原花青素的含量,并进行了高效液相色谱(HPLC)分析。通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基法测定抗氧化活性。使用无水乙醇的索氏提取法可获得高产率的酚类化合物。此外,花青素提取物显示出高水平的花青素含量。液相色谱分析表明黄酮类化合物占比很高。索氏提取物在清除自由基方面最有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a135/12203400/8a997b860387/FSN3-13-e70521-g001.jpg

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