Laboratoire Des Plantes Extrêmophile, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia; Univ. Bordeaux2, ISVV, Groupe d'Etude des Substances Végétales à Activité Biologique, EA 3675, F-33882 Villenave d'Ornon, France.
Food Chem. 2014 Mar 1;146:466-71. doi: 10.1016/j.foodchem.2013.09.077. Epub 2013 Sep 25.
Limoniastramide, a new dimer of phenolic acid amide, isolated from Limoniastrum guyonianum, along with two natural monomers N-E-caffeoyl tyramine (1) and N-E-feruloyl tyramine (2), using centrifugal partition chromatography (CPC). Their structures were determined on the basis of spectroscopic data analysis. We investigate the antioxidant activities of Limoniastrum amides using various in vitro assays. Results showed that N-E-feruloyl tyramine and N-E-caffeoyl tyramine exhibited the highest DPPH scavenging activity compared to the dimer (IC50=0.5, 0.6 and 6.5μg/ml, respectively). In addition, they have significant capacities to inhibit the bleaching of β-carotene. Limoniastramide presented the best activity with an IC50 value equal to 8μg/ml. Finally, the N-E-caffeoyl tyramine showed the highest reducing power (EC50=26μg/ml) compared to the other molecules. The present study found that L. guyonianum amides have effective in vitro antioxidant and radical scavenging activity which can be used in pharmacological and food industry due to their antioxidant properties.
从黄花木中分离得到的新二聚体酚酸酰胺Limoniastramide，以及两种天然单体 N-E-咖啡酰酪胺（1）和 N-E-阿魏酰酪胺（2），采用离心分配色谱（CPC）。根据光谱数据分析确定它们的结构。我们使用各种体外测定法研究了黄花木酰胺的抗氧化活性。结果表明，与二聚体相比，N-E-阿魏酰酪胺和 N-E-咖啡酰酪胺具有最高的 DPPH 清除活性（IC50=0.5、0.6 和 6.5μg/ml）。此外，它们具有抑制β-胡萝卜素漂白的显著能力。Limoniastramide 的活性最好，IC50 值等于 8μg/ml。最后，与其他分子相比，N-E-咖啡酰酪胺具有最高的还原能力（EC50=26μg/ml）。本研究发现，黄花木酰胺具有有效的体外抗氧化和自由基清除活性，由于其抗氧化特性，可用于药理学和食品工业。
