Bouchouka Elmouloud, Djilani Abdelouaheb, Bekkouche Abdesselem
LSBO, Department of Chemistry, Annaba University, Algeria.
Acta Sci Pol Technol Aliment. 2012 Jan-Mar;11(1):61-5.
Saharan plants are known by their high content of antioxidant products like phenolic compounds due to the extreme climatic conditions. They constitute the basis of treatments used by local population for various diseases. The purposes of this study were to measure the total phenolic compounds and total fl avonoid compounds, to determine antioxidant capacity, and to evaluate the antibacterial activity of three wild Saharan medicinal plants.
Hexane and ethyl acetate fractions of ethanol:water extract and the residu of the extracted aqueous layer of Ferula vesceritensis fruits, Genista saharae aerial parts and Zilla macropterae fruits were assayed to determine their antibacterial activity using the disc diffusion method against: Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853). In addition, the total phenolic compounds and total flavonoids and antioxydant activity using DPPH test of ethyl acetate fractions (EAF) of plant parts studied were investigated. Gallic acid, quercetin and vitamin C were used for these parameters.
Among the extracts tested, ethyl acetate fractions of all plants and hexane fraction of F. vesceritensis showed activity against S. aureus. Good activity was shown by EAF of G. saharae. According to the results, it is observed that Z. macropterae fruits possess a good antioxidant activity.
The results indicate that the ethyl acetate fraction of G. sahara Aerial parts possesses a good antibacterial activity against S. aureus, which justifies its use in traditional medicine for treating respiratory diseases. Furthermore, evaluation of in vitro antioxidant capacity of Ethyl acetate fractions of these plants, particular Z. macroptera fruits, has also provided interesting results. Zilla macroptera fruits may therefore be a good source of antioxidants.
由于极端气候条件，撒哈拉沙漠植物以其富含酚类化合物等抗氧化产物而闻名。它们构成了当地居民用于治疗各种疾病的疗法基础。本研究的目的是测定三种撒哈拉沙漠野生药用植物的总酚类化合物和总黄酮类化合物含量，确定其抗氧化能力，并评估其抗菌活性。
采用纸片扩散法，对阿魏（Ferula vesceritensis）果实、撒哈拉染料木（Genista saharae）地上部分和大翅苣（Zilla macropterae）果实的乙醇：水提取物的己烷和乙酸乙酯馏分以及提取水层的残渣进行抗菌活性测定，受试菌株为：大肠杆菌（ATCC 25922）、金黄色葡萄球菌（ATCC 25923）和铜绿假单胞菌（ATCC 27853）。此外，还研究了所研究植物部分的乙酸乙酯馏分（EAF）的总酚类化合物、总黄酮类化合物及采用DPPH法测定的抗氧化活性。这些参数测定使用没食子酸、槲皮素和维生素C作为对照。
在所测试的提取物中，所有植物的乙酸乙酯馏分和阿魏的己烷馏分对金黄色葡萄球菌均表现出活性。撒哈拉染料木的乙酸乙酯馏分显示出良好活性。根据结果观察到，大翅苣果实具有良好的抗氧化活性。
结果表明，撒哈拉染料木地上部分的乙酸乙酯馏分对金黄色葡萄球菌具有良好的抗菌活性，这证明了其在传统医学中用于治疗呼吸道疾病的合理性。此外，对这些植物特别是大翅苣果实的乙酸乙酯馏分的体外抗氧化能力评估也得出了有趣的结果。因此，大翅苣果实可能是抗氧化剂的良好来源。
