Guo Li-ke, Zhang Chao-xian, Guo Xiao-feng
Department of Stomatology,the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2012 Aug;34(4):390-5. doi: 10.3881/j.issn.1000-503X.2012.04.015.
To investigate the association of the polymorphisms of aldehyde dehydrogenase-2(ALDH2) and CYP2E1-RsaI genes and alcohol consumption with oral squamous cell carcinoma (OSCC).
The genetic polymorphisms of ALDH2 and CYP2E1-RsaI were determined by polymorphism-polymerase chain reaction (PCR) technique in the peripheral blood leukocytes of 320 OSCC patients and 320 non-cancer controls.
The frequencies of ALDH2 variant genotypes and CYP2E1-RsaI (c2/c2) were 70.94% and 39.06% in the OSCC group and 43.44% and 20.62% in the control group (both P<0.01). The risk of OSCC with ALDH2 variant genotypes was significantly higher than that in control group (OR=3.178, 95% CI=1.917-4.749), whereas the subjects carried with CYP2E1-RsaI (c2/c2) also had a high risk of OSCC (OR=2.467, 95%CI=1.783-4.045). Combined analysis of the polymorphisms showed that percentage of ALDH2 variant genotypes/CYP2E1-RsaI (c2/c2) in OSCC group and control group was 32.19% and 6.25%, respectively (P<0.01). Carriers of ALDH2 variant genotypes/CYP2E1-RsaI (c2/c2) had a high risk of OSCC (OR=9.792, 95%CI=3.583-12.472). The percentage of alcohol consumption was significantly higher in OSCC group than in the control group (OR=2.861, 95% CI=1.541-4.781, P<0.01), and ALDH2 variant genotypes and CYP2E1-RsaI (c2/c2) showed synergic effects with alcohol consumption for the increased risk of OSCC (OR=41.152, 95%CI=19.903-67.551).
The polymorphisms of ALDH2 and CYP2E1-RsaI genes and alcohol consumption, independently and synergically, increase the risk of OSCC.
探讨乙醛脱氢酶2（ALDH2）和细胞色素P450 2E1-RsaI基因多态性及饮酒与口腔鳞状细胞癌（OSCC）的关系。
采用多态性聚合酶链反应（PCR）技术检测320例OSCC患者和320例非癌对照者外周血白细胞中ALDH2和CYP2E1-RsaI的基因多态性。
OSCC组中ALDH2变异基因型和CYP2E1-RsaI（c2/c2）的频率分别为70.94%和39.06%，对照组分别为43.44%和20.62%（均P<0.01）。携带ALDH2变异基因型的OSCC患者风险显著高于对照组（OR=3.178，95%CI=1.917-4.749），而携带CYP2E1-RsaI（c2/c2）的个体患OSCC风险也较高（OR=2.467，95%CI=1.783-4.045）。多态性联合分析显示，OSCC组和对照组中ALDH2变异基因型/CYP2E1-RsaI（c2/c2）的比例分别为32.19%和6.25%（P<0.01）。携带ALDH2变异基因型/CYP2E1-RsaI（c2/c2）的个体患OSCC风险较高（OR=9.792，95%CI=3.583-12.472）。OSCC组饮酒比例显著高于对照组（OR=2.861，95%CI=1.541-4.781，P<0.01），且ALDH2变异基因型和CYP2E1-RsaI（c2/c2）与饮酒对OSCC风险增加具有协同作用（OR=41.152，95%CI=19.903-67.551）。
ALDH2和CYP2E1-RsaI基因多态性及饮酒单独或协同增加OSCC风险。
