Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
J Ethnopharmacol. 2012 Oct 31;144(1):22-32. doi: 10.1016/j.jep.2012.08.014. Epub 2012 Aug 31.
Seeds of Centratherum anthelminticum (Asteraceae) have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders. Folk medicine from Rayalaseema (Andhra Pradesh, India) reported wide spread usage in diabetes.
To investigate the hypoglycemic properties and mechanism of the methanolic fraction of C. anthelminticum seeds (CAMFs) on mouse β-TC6 pancreatic cell line and streptozotocin (STZ)-induced diabetic rat models.
We investigated the crude methanolic fraction of C. anthelminticum seeds (CAMFs) on β-TC6 cell line and confirmed its effects on type 1 and type 2 diabetic rats to understand its mechanism in managing diabetes mellitus. CAMFs were initially tested on β-TC6 cells for cytotoxicity, 2-NBDG glucose uptake, insulin secretion and glucose transporter (GLUT-1, 2 and 4) protein expression. Furthermore, streptozotocin (STZ)-induced type 1 diabetic and STZ-nicotinamide-induced type 2 diabetic rats were intraperitoneally (i.p) injected or administered orally with CAMFs daily for 28 days. The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated.
In cell line studies, CAMFs showed non-cytotoxic effect on β-TC6 cell proliferation compared to untreated control cells at 50 μg/ml. CAMFs increased glucose uptake and insulin secretion dose-dependently by up-regulating GLUT-2 and GLUT-4 expression in these cells. Further in vivo studies on streptozotocin induced diabetic rat models revealed that CAMFs significantly reduced hyperglycemia by augmenting insulin secretion in type 2 diabetic rats. However, CAMFs displayed less significant effects on type 1 diabetic rats.
CAMFs demonstrated anti-diabetic potential on β-TC6 cells and type 2 diabetic rat model, plausibly through enhancing glucose uptake and insulin secretion.
Centratherum anthelminticum（菊科）的种子在印度草医学中被广泛用于治疗糖尿病和皮肤疾病。来自印度安得拉邦 Rayalaseema 的民间医学报告称，该种子在糖尿病治疗方面有广泛的应用。
研究 Centratherum anthelminticum 种子的甲醇提取物（CAMFs）对小鼠β-TC6 胰腺细胞系和链脲佐菌素（STZ）诱导的糖尿病大鼠模型的降血糖作用及其机制。
我们研究了 Centratherum anthelminticum 种子的粗甲醇提取物（CAMFs）对β-TC6 细胞的作用，并证实了其对 1 型和 2 型糖尿病大鼠的影响，以了解其在糖尿病管理中的机制。CAMFs 最初在β-TC6 细胞上进行了细胞毒性、2-NBDG 葡萄糖摄取、胰岛素分泌和葡萄糖转运蛋白（GLUT-1、2 和 4）蛋白表达的检测。此外，链脲佐菌素（STZ）诱导的 1 型糖尿病和 STZ-烟酰胺诱导的 2 型糖尿病大鼠通过腹腔内（i.p）注射或口服每日给予 CAMFs 治疗 28 天。随后评估 CAMFs 对血糖和胰岛素水平的影响。
在细胞系研究中，与未经处理的对照细胞相比，CAMFs 在 50μg/ml 时对β-TC6 细胞增殖没有细胞毒性作用。CAMFs 通过上调这些细胞中的 GLUT-2 和 GLUT-4 表达，呈剂量依赖性地增加葡萄糖摄取和胰岛素分泌。进一步在链脲佐菌素诱导的糖尿病大鼠模型中的体内研究表明，CAMFs 通过增强 2 型糖尿病大鼠的胰岛素分泌显著降低高血糖。然而，CAMFs 在 1 型糖尿病大鼠中显示出不那么显著的效果。
CAMFs 在β-TC6 细胞和 2 型糖尿病大鼠模型中显示出抗糖尿病潜力，可能是通过增强葡萄糖摄取和胰岛素分泌。
