Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China.
Department of Nutrition, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Oxid Med Cell Longev. 2018 Dec 2;2018:2917387. doi: 10.1155/2018/2917387. eCollection 2018.
Oxidative stress and inflammation play an important role in hypertensive animals and patients. Hydrogen plays a role of antioxidation and anti-inflammation. Calcium and magnesium play an important role in reducing hypertension and antioxidant. Filtered water contains abundant hydrogen and a large number of other essential elements of the human body. We investigated the protective effects of filtered water on hypertensive mice. To establish hypertension model, ICR mice were administered with N'-nitro-L-arginine methyl ester (L-NAME) hydrochloride 64 mg/kg per day for 1 month. The hypertensive mice were, respectively, administered with pure water, tap water, and filtered water for 2 months. Lipid peroxidation, antioxidant enzymatic activity, endothelin-1 (ET-1), angiotensin II (Ang II), and proinflammatory cytokines (TNF-, IL-6, and IL-1) were assessed. Expressions of phosphorylated NF-B P65 in the kidney were analyzed by western blot. qRT-PCR analysis was adopted to determine the expression levels of the proinflammatory cytokines and NF-B P65. The results demonstrated that filtered water can reduce the blood pressure. Filtered water treatment restored the activity of antioxidant enzymes, downregulated ET-1, and Ang II in the serum of mice. Filtered water treatment suppressed proinflammatory cytokines and decreased the mRNA expression of TNF-, IL-6, IL-1, and NF-B P65. Consumption of filtered water inhibited the expression of NF-B P65. This suggests that filtered water can reduce the blood pressure. The protection mechanisms include downregulating oxidative stress and inhibiting inflammation, which is partly due to the inhibition of the NF-B signaling pathway.
氧化应激和炎症在高血压动物和患者中起着重要作用。氢气具有抗氧化和抗炎作用。钙和镁在降低高血压和抗氧化方面起着重要作用。过滤水含有丰富的氢气和大量人体必需的其他元素。我们研究了过滤水对高血压小鼠的保护作用。为了建立高血压模型，我们每天给 ICR 小鼠服用 N'-硝基-L-精氨酸甲酯（L-NAME）盐酸盐 64mg/kg，持续 1 个月。然后，将高血压小鼠分别用纯水、自来水和过滤水喂养 2 个月。评估了脂质过氧化、抗氧化酶活性、内皮素-1（ET-1）、血管紧张素 II（Ang II）和促炎细胞因子（TNF-α、IL-6 和 IL-1）。通过 Western blot 分析肾脏中磷酸化 NF-B P65 的表达。采用 qRT-PCR 分析确定促炎细胞因子和 NF-B P65 的表达水平。结果表明，过滤水可以降低血压。过滤水治疗恢复了抗氧化酶的活性，下调了血清中 ET-1 和 Ang II 的水平。过滤水治疗抑制了促炎细胞因子的产生，并降低了 TNF-α、IL-6、IL-1 和 NF-B P65 的 mRNA 表达。过滤水的摄入抑制了 NF-B P65 的表达。这表明过滤水可以降低血压。保护机制包括下调氧化应激和抑制炎症，部分原因是抑制了 NF-B 信号通路。
