State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory for Microbial and Plant Genetic Engineering, Ministry of Education, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
J Proteomics. 2013 Jan 14;78:221-30. doi: 10.1016/j.jprot.2012.08.013. Epub 2012 Aug 29.
The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus-host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially expressed protein spots were identified in the purified vesicle protein samples by two-dimensional electrophoresis and mass spectrometry. Down-regulated proteins were mostly cargo proteins involved in primary metabolism and energy generation and up-regulated proteins were mostly vesicle associated proteins and ABC transporter. A virus-encoded protein p48 was found to have four forms with different molecular mass in vesicles from the virus-infected strain. While a few of the randomly selected differentially expressed proteins were in accordance with their transcription profiles, majority were not in agreement with their mRNA accumulation patterns, suggesting that an extensive post-transcriptional regulation may have occurred in the host fungus upon a hypovirus infection.
栗疫病菌（Cryphonectria parasitica）及其伴生的病毒构成了研究真菌发病机制和真菌病毒-宿主相互作用的模式系统。迄今为止，该领域的知识主要来自于基因转录水平的研究。在这里，我们报道了对伴生病原菌和感染病毒的宿主真菌的囊泡蛋白进行的系统分析。通过二维电泳和质谱分析，在纯化的囊泡蛋白样品中鉴定出 33 个差异表达的蛋白斑点。下调表达的蛋白大多是参与初级代谢和能量产生的货物蛋白，而上调表达的蛋白大多是囊泡相关蛋白和 ABC 转运蛋白。在病毒感染株的囊泡中发现了一种病毒编码的 p48 蛋白，它有四种不同分子量的形式。虽然随机选择的一些差异表达蛋白与其转录谱一致，但大多数与它们的 mRNA 积累模式不一致，这表明在宿主真菌感染病毒后，可能发生了广泛的转录后调控。
