State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory for Microbial and Plant Genetic Engineering, Ministry of Education, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Sci Rep. 2016 Oct 4;6:34308. doi: 10.1038/srep34308.
To understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identify and quantify the secreted proteins. A total of 403 unique proteins were identified from the secretome of the wild type virus-free strain EP155. Of these proteins, 329 were predicted to be involved in known secretory pathways and they are primarily composed of metabolic enzymes, biological regulators, responders to stimulus and components involved in plant-pathogen interactions. When infected with the hypovirus CHV1-EP713, 99 proteins were found to be differentially expressed as compared to the wild type strain EP155. These proteins were mainly related to plant cell wall degradation, response to host defense, fungal virulence and intracellular structure. The effects of CHV1 on secreted proteins may reveal a relationship between physiological pathways and hypovirulence.
为了了解类病毒感染对栗疫病菌（Cryphonectria parasitica）分泌组的影响，栗疫病菌是一种植物病原丝状真菌，我们采用二维电泳（2-DE）和同位素相对和绝对定量标记（iTRAQ）技术来鉴定和定量分泌蛋白。从野生型无病毒株 EP155 的分泌组中鉴定出了 403 种独特的蛋白质。其中，329 种被预测参与已知的分泌途径，它们主要由代谢酶、生物调节剂、对刺激的响应者以及参与植物-病原体相互作用的成分组成。当感染类病毒 CHV1-EP713 时，与野生型菌株 EP155 相比，发现有 99 种蛋白质表达差异。这些蛋白质主要与植物细胞壁降解、对宿主防御的反应、真菌毒力和细胞内结构有关。CHV1 对分泌蛋白的影响可能揭示了生理途径与类病毒弱毒之间的关系。
