Laboratório de Sistemática Bioquímica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, CEP 21040-360, Brazil.
Trans R Soc Trop Med Hyg. 2012 Nov;106(11):683-7. doi: 10.1016/j.trstmh.2012.07.007. Epub 2012 Sep 3.
The genetic polymorphism of Leishmania (Viannia) braziliensis detected in cases of mucosal leishmaniasis (ML) from HIV-infected and non HIV-infected patients was evaluated. Nine samples from three HIV-infected patients and five samples from five non HIV-infected patients were analysed by polymerase chain reaction (PCR), low-stringency single-specific primer PCR (LSSP-PCR) and phenetic analysis. The presence of L. (V.) braziliensis DNA was detected in all samples by specific PCR assay. The intraspecific polymorphism of the variable region of L. (V.) braziliensis kDNA minicircles was investigated by LSSP-PCR. Phenetic analysis grouped the genetic profiles into two distinct clusters, which discriminated between samples obtained from HIV-infected and non HIV-infected patients. In two HIV-infected patients, identical genetic profiles were detected in lesions biopsied at different times after the treatment of the initial lesion. Interestingly, genetically divergent profiles were detected in the cutaneous and mucosal lesions of the same HIV-infected patient collected at the same time. This is the first work comparing genetic polymorphism of L. (V.) braziliensis in cases of mucosal leishmaniasis from HIV-infected and non HIV-infected patients.
评估了 HIV 感染和非 HIV 感染患者黏膜利什曼病（ML）病例中检测到的巴西利什曼原虫（Viannia）遗传多态性。通过聚合酶链反应（PCR）、低严格性单特异性引物 PCR（LSSP-PCR）和表型分析对来自 3 名 HIV 感染患者的 9 个样本和来自 5 名非 HIV 感染患者的 5 个样本进行了分析。特异性 PCR 检测到所有样本中均存在 L.（V.）巴西利什曼原虫 DNA。通过 LSSP-PCR 研究了 L.（V.）巴西利什曼原虫 kDNA 微环的种内变异区的遗传多态性。表型分析将遗传谱分为两个不同的聚类，可区分 HIV 感染和非 HIV 感染患者的样本。在两名 HIV 感染患者中，在初始病变治疗后不同时间采集的病变活检中检测到相同的遗传谱。有趣的是，在同一时间采集的同一 HIV 感染患者的皮肤和黏膜病变中检测到遗传上不同的谱。这是首次比较 HIV 感染和非 HIV 感染患者黏膜利什曼病中巴西利什曼原虫遗传多态性的研究。
