Guo Yunzhong, Li Luhong, Tan Lihua, Tang Xiaohong, Yang Qiong, Jiang Weihong
Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Aug;37(8):829-33. doi: 10.3969/j.issn.1672-7347.2012.08.012.
To investigate the impact of renal denervation on the blood pressure, plasma renalase content and expression of renalase and tyrosine hydroxylase (TH) in the idney of spontaneous hypertensive (SH) rats and to explore the role of renal denervation in lowering the blood pressure.
SH rats were randomly assigned into a baseline group, a surgery (renal denervation) group, a sham group and a control group (n=48). WKY rats matched in age (n=12) served as a baseline control group. All rats were housed until 12 weeks old. Then, the rats in the baseline group and the WKY group were sacrificed whose blood and kidney were collected for examination. In the renal denervation group, the sham group and the control group, the blood pressure was monitored continuously. One week and 6 weeks after the renal denervation, 6 rats in each group were sacrificed whose blood and kidney were collected. ELISA was employed to measure the plasma renalase and Western blot assay done to detect the expression of TH and renalase in the kidney.
Compared with WKY rats, blood pressure significantly increased and TH protein expression markedly elevated (P<0.05) in SH rats in the baseline group, but plasma renalase content and protein expression of renalase in the kidney dramatically reduced (P<0.05). One week after the surgery, the mean arterial pressure and TH protein expression in the surgery group were lowered compared with the baseline group and dramatically reduced compared with the sham group and the control group (P<0.05). In the surgery group, the renalase level was markedly increased compared with the baseline group, the sham group, and the control group (P<0.05). Six weeks after the renal denervation, the mean arterial pressure and TH level in the surgery group were significantly increased but the renalase content and expression markedly reduced compared with those 1 week, but there were no marked differences among the surgery group, the sham group, and the control group (P>0.05). No pronounced differences in the above variables were found between the sham group and the control group at any time point (P>0.05).
Renal denervation can lower the blood pressure, which may attribute to the suppression of sympathetic nerves, increase in plasma renalase content and renalase expression in the kidney.
探讨肾去神经支配对自发性高血压（SH）大鼠血压、血浆肾酶含量以及肾脏中肾酶和酪氨酸羟化酶（TH）表达的影响，并探讨肾去神经支配在降低血压中的作用。
将SH大鼠随机分为基线组、手术（肾去神经支配）组、假手术组和对照组（n = 48）。选取年龄匹配的WKY大鼠（n = 12）作为基线对照组。所有大鼠饲养至12周龄。然后，处死基线组和WKY组大鼠，采集血液和肾脏进行检测。在肾去神经支配组、假手术组和对照组中，持续监测血压。肾去神经支配后1周和6周，每组处死6只大鼠，采集血液和肾脏。采用酶联免疫吸附测定（ELISA）法检测血浆肾酶，采用蛋白质免疫印迹法检测肾脏中TH和肾酶的表达。
与WKY大鼠相比，基线组SH大鼠血压显著升高，TH蛋白表达明显升高（P < 0.05），但血浆肾酶含量和肾脏中肾酶蛋白表达显著降低（P < 0.05）。手术后1周，手术组平均动脉压和TH蛋白表达较基线组降低，与假手术组和对照组相比显著降低（P < 0.05）。手术组肾酶水平与基线组、假手术组和对照组相比显著升高（P < 0.05）。肾去神经支配6周后，手术组平均动脉压和TH水平较1周时显著升高，但肾酶含量和表达显著降低，与假手术组和对照组相比无显著差异（P > 0.05）。在任何时间点，假手术组和对照组上述变量均无明显差异（P > 0.05）。
肾去神经支配可降低血压，这可能归因于交感神经的抑制、血浆肾酶含量的增加以及肾脏中肾酶表达的增加。
