Hebei Key Laboratory of Pharmaceutic Molecular Chemistry, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang 050018, People's Republic of China.
Appl Biochem Biotechnol. 2012 Nov;168(6):1394-404. doi: 10.1007/s12010-012-9865-7. Epub 2012 Sep 7.
During the two-stage submerged fermentation of medicinal mushroom Cordyceps militaris, it was found that K(+), Ca(2+), Mg(2+), and Mn(2+) were favorable to the mycelial growth. The EPS production reached the highest levels in the media containing Mg(2+) and Mn(2+). However, Ca(2+) and K(+) almost failed to increase significantly exopolysaccharides (EPS) production. Sodium dodecyl sulfate (SDS) significantly enhanced EPS production compared with that of without adding SDS when SDS was added on static culture stage of two-stage cultivation process. The presence of Tween 80 in the medium not only simulated mycelial growth but also increased EPS production. By response surface methods (RSM), EPS production reached its peak value of 3.28 g/L under optimal combination of 27.6 mM Mg(2+), 11.1 mM Mn(2+), and 0.05 mM SDS, which was 3.76-fold compared with that of without metal ion and surfactant. The results obtained were useful in better understanding the regulation for efficient production of EPS of C. militaris in the two-stage submerged culture.
在药用真菌蛹虫草的两段式发酵过程中，发现 K(+)、Ca(2+)、Mg(2+)和 Mn(2+)有利于菌丝生长。在含有 Mg(2+)和 Mn(2+)的培养基中，EPS 产量达到最高水平。然而，Ca(2+)和 K(+)几乎未能显著增加胞外多糖 (EPS)的产量。与不加 SDS 的对照相比，在两段培养过程的静置培养阶段添加十二烷基硫酸钠 (SDS)时，SDS 显著提高了 EPS 的产量。在培养基中加入吐温 80 不仅模拟了菌丝生长，而且增加了 EPS 的产量。通过响应面法 (RSM)，在最佳组合 27.6mM Mg(2+)、11.1mM Mn(2+)和 0.05mM SDS 下，EPS 产量达到 3.28g/L 的峰值，与无金属离子和表面活性剂的对照相比，提高了 3.76 倍。这些结果有助于更好地理解蛹虫草两段式发酵过程中胞外多糖高效生产的调控机制。
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