Mombelli A, Buser D, Lang N P, Berthold H
University of Berne, School of Dental Medicine, Switzerland.
J Clin Periodontol. 1990 Jan;17(1):48-54. doi: 10.1111/j.1600-051x.1990.tb01047.x.
29 periodontally healthy subjects (11 female and 18 male) with a mean age of 24 years (range 19 to 38 years) and with partially erupted lower third molars participated in this study. 18 subjects demonstrated no signs or symptoms of acute inflammation and were without pain (group A). 5 subjects showed redness of the pericoronal tissues and experienced pain upon palpation (group B). 6 subjects suffered from acute pain and exhibited formation of pus (group C). Microbiological samples were taken from the lateral aspect of the pericoronal space using the paperpoint-method. Continuous anaerobic techniques were utilized for microbiological processing. The samples were cultivated on ETSA and on selective media and were studied by darkfield microscopy. Gram-negative anaerobic rods accounted for 27% (group A), 34% (group B) and 39% (group C) of all organisms growing on ETSA. Bacteroides intermedius was detected in 61% (group A), 80% (group B) and 83% (group C) of the samples. B. gingivalis was found in 1 sample of group A only. Fusobacterium sp. was detected in 56% (group A), 80% (group B) and 33% (group C) of the samples. Capnocytophaga were seen in 67% (group A), 20% (group B) and 50% of the samples. Actinobacillus actinomycetemcomitans was found in 44% (group A), 40% (group B) and 17% (group C). 72% of the group A and 100% of the group B and C samples contained spirochetes. In all of those positive samples, small spirochetes were present, but only 78% contained medium and only 48% large spirochetes.(ABSTRACT TRUNCATED AT 250 WORDS)
29名牙周健康受试者（11名女性和18名男性）参与了本研究，他们的平均年龄为24岁（年龄范围19至38岁），下颌第三磨牙部分萌出。18名受试者无急性炎症的体征或症状，且无疼痛（A组）。5名受试者冠周组织发红，触诊时疼痛（B组）。6名受试者遭受急性疼痛并出现脓肿形成（C组）。使用纸尖法从冠周间隙外侧采集微生物样本。采用连续厌氧技术进行微生物处理。样本在ETSA和选择性培养基上培养，并通过暗视野显微镜进行研究。在ETSA上生长的所有微生物中，革兰氏阴性厌氧杆菌在A组占27%，B组占34%，C组占39%。中间普氏菌在A组61%、B组80%和C组83%的样本中被检测到。牙龈卟啉单胞菌仅在A组的1个样本中被发现。梭杆菌属在A组56%、B组80%和C组33%的样本中被检测到。二氧化碳嗜纤维菌在A组67%、B组20%和50%的样本中被观察到。伴放线放线杆菌在A组44%、B组40%和C组17%的样本中被发现。A组72%、B组和C组100%的样本含有螺旋体。在所有这些阳性样本中，均存在小螺旋体，但仅78%含有中螺旋体，仅48%含有大螺旋体。（摘要截断于250字）
