荜茇粗提物对耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌、霍乱弧菌和肺炎链球菌的抗菌活性。

Antimicrobial activity of the crude extract of Piper sarmentosum against methicilin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Vibrio cholera and Streptococcus pneumoniae.

机构信息

Institute for Research in Molecular Medicine (INFORMM) University Sains Malaysia, 11800 Penang, Malaysia.

出版信息

Eur Rev Med Pharmacol Sci. 2012 Jul;16 Suppl 3:105-11.

Abstract

BACKGROUND AND AIMS

The emergence of novel diseases caused by microbial pathogens and the undesirable side effects of certain antibiotics has been a recent dilemma in the medical arena. Consequently, it has stirred the discovery of many naturally occurring agents which could possibly provide important ramifications against various pharmacological targets and to combat various ailments. The main aim of the present study was to determine the antimicrobial activity of the crude methanolic extract of Piper (P.) sarmentosum against methicillin resistant Staphylococcus aureus (MRSA), Escherichia coli, Vibrio cholera and Streptococcus pneumoniae.

MATERIALS AND METHODS

The plant materials were extracted by percolation in 70% methanol. Only the leaves were used in this study. Initial antimicrobial screening using disc diffusion assay was conducted and further screening of the antimicrobial properties in the plant was performed using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The phytochemical constituents in the plant were evaluated via phytochemical screening and thin layer chromatography (TLC).

RESULTS

P. sarmentosum inhibited the growth of MRSA with an inhibition zone of 10.0 mm. There was no inhibition zone observed for the other microbes tested. MIC test showed a value of 50mg/ml and MBC results showed no Colony Forming Unit (CFU) at 100 mg/ml against MRSA. Phytochemical screening of the crude extract indicated the presence of tannins, flavonoids, alkaloids glycosides and anthraquinone. Thin-layer chromatography (TLC) further confirmed the presence of flavonoids and alkaloids in the extract.

CONCLUSION

P. sarmentosum has shown to have some antimicrobial properties against MRSA. Based on the MIC index (MBC/MIC), the extract exhibits bactericidal effects against MRSA. TLC analysis indicated the presence of flavonoids and alkaloids which could have contributed to the antimicrobial activity of the plant extract.

摘要

背景与目的

新型微生物病原体疾病的出现以及某些抗生素的不良副作用是医学领域的一个近期困境。因此，这激发了人们对许多天然存在的物质的发现，这些物质可能对各种药理学靶点具有重要意义，并能对抗各种疾病。本研究的主要目的是测定荜茇（Piper（P.）sarmentosum）粗甲醇提取物对耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌、霍乱弧菌和肺炎链球菌的抗菌活性。

材料与方法

采用渗漉法用 70%甲醇提取植物材料。本研究仅使用叶片。采用纸片扩散法进行初步抗菌筛选，采用最低抑菌浓度（MIC）和最低杀菌浓度（MBC）进一步筛选植物的抗菌特性。采用植物的化学成分筛选和薄层色谱（TLC）评估植物中的植物化学成分。

结果

荜茇对 MRSA 的生长有抑制作用，抑菌圈直径为 10.0mm。对其他测试的微生物没有观察到抑菌圈。MIC 试验表明，对 MRSA 的 MIC 值为 50mg/ml，MBC 结果表明，在 100mg/ml 时没有形成 CFU。粗提物的化学成分筛选表明存在单宁、类黄酮、生物碱糖苷和蒽醌。薄层层析（TLC）进一步证实了提取物中存在类黄酮和生物碱。