Karupiah G, Coupar B E, Andrew M E, Boyle D B, Phillips S M, Müllbacher A, Blanden R V, Ramshaw I A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
J Immunol. 1990 Jan 1;144(1):290-8.
The role of cytotoxic T cells and NK cells in the recovery of immunodeficient, athymic, nude mice infected with a recombinant vaccinia virus (VV) encoding murine IL-2 was investigated. Kinetic studies with the IL-2-encoding recombinant (VV-HA-IL2) and control (VV-HA-TK) viruses excluded a role for cytotoxic T cells but suggested the possible involvement of NK cells. In athymic nude mice given VV-HA-IL2, NK activity was at least threefold higher than mice infected with VV-HA-TK and this activity persisted for at least 6 days after infection. The effectors mediating the NK-like activity were asialo-GM1+ (as-GM1+), Thy1.2+/-, CD4- and CD8-, the phenotype of conventional NK cells. Elevated NK activity coincided with the rapid clearance of VV-HA-IL2 from ovaries of infected normal CBA/H mice but not from ovaries of CBA beige mice which had no detectable NK activity in spleens or ovaries. The expression of IL-2 in recombinant VV infection probably induces a cascade of immunologic effects of which elevated NK activity is one. We speculate that the chemoattractant and NK activity augmenting effects of IL-2 may contribute to recovery from VV-infection.
研究了细胞毒性T细胞和自然杀伤（NK）细胞在感染编码小鼠白细胞介素-2（IL-2）的重组痘苗病毒（VV）的免疫缺陷、无胸腺裸鼠恢复过程中的作用。对编码IL-2的重组病毒（VV-HA-IL2）和对照病毒（VV-HA-TK）进行的动力学研究排除了细胞毒性T细胞的作用，但提示NK细胞可能参与其中。在给予VV-HA-IL2的无胸腺裸鼠中，NK活性比感染VV-HA-TK的小鼠至少高三倍，且这种活性在感染后至少持续6天。介导类NK活性的效应细胞是无唾液酸GM1+（as-GM1+）、Thy1.2+/-、CD4-和CD8-，这是传统NK细胞的表型。NK活性升高与感染正常CBA/H小鼠卵巢中VV-HA-IL2的快速清除同时发生，但在脾脏或卵巢中无可检测到的NK活性的CBA米色小鼠卵巢中未出现这种情况。重组VV感染中IL-2的表达可能诱导一系列免疫效应，其中NK活性升高是其中之一。我们推测，IL-2的趋化作用和增强NK活性的作用可能有助于从VV感染中恢复。
