Entomology Department, Cairo University, Giza 12613, Egypt.
J Insect Sci. 2011;11:177. doi: 10.1673/031.011.17701.
The genetic structure of the Egyptian peach fruit fly (Bactrocera zonata (Saunders) (Diptera: Tephritidae)) population was analyzed using total RNA from adult females. A portion of mitochondrial cytochrome oxidase I (COI), 369 bp was amplified using RT-PCR, and was sequenced and analyzed to clarify the phylogenetic relationship of B. zonata established in Egypt. The data suggested that the gene shared a similarity in sequence compared to Bactrocera COI gene found in GenBank. Molecular phylogenetic analyses were performed based on nucleotide sequences in order to examine the position of the Egyptian population among many other species of fruit flies. The results indicate that four accession numbers of B. zonata (three from New Zealand and one from India) are closely related, while the Egyptian B. zonata are close to the 71 accession numbers of Bactrocera include one B. zonata from New Zealand. These two B. zonata from Egypt and New Zealand showed a close relationship in neighbor-joining analysis using the seven accession numbers of B. zonata. In addition, a theoretical restriction map of the homology portion of the COI gene was constructed using 212 restriction enzymes obtained from the restriction enzyme database to identify the Egyptian and New Zealand B. zonata.
采用来自成年雌蝇的总 RNA 分析埃及桃果实蝇（Bactrocera zonata（Saunders）（双翅目：瘿蚊科））种群的遗传结构。使用 RT-PCR 扩增了 369 bp 的线粒体细胞色素氧化酶 I（COI）的一部分，并对其进行测序和分析，以阐明在埃及建立的 B. zonata 的系统发育关系。数据表明，该基因与 GenBank 中发现的 Bactrocera COI 基因在序列上具有相似性。基于核苷酸序列进行了分子系统发育分析，以检查埃及种群在许多其他果蝇种中的位置。结果表明，有四个 B. zonata 的登录号（三个来自新西兰，一个来自印度）密切相关，而埃及的 B. zonata 则与包括一个来自新西兰的 B. zonata 在内的 71 个 Bactrocera 登录号密切相关。这两个来自埃及和新西兰的 B. zonata 在使用七个 B. zonata 的登录号的邻接法分析中显示出密切的关系。此外，使用从限制酶数据库中获得的 212 种限制酶构建了 COI 基因同源部分的理论限制图谱，以鉴定埃及和新西兰的 B. zonata。
