Jamnongluk Wanwisa, Baimai Visut, Kittayapong Pattamaporn
Center for Vectors and Vector-borne Diseases and Department of Biology, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand.
Genome. 2003 Feb;46(1):112-8. doi: 10.1139/g02-113.
We compared sequences of the mitochondrial cytochrome oxidase I gene of eight species of the Bactrocera tau complex using Bactrocera dorsalis, Bactrocera pyrifoliae, Ceratitis capitata, Anopheles gambiae, and Locusta migratoria as outgroups. A 639-bp variable region was sequenced. The sequence divergence between species in the B. tau complex ranged from 0.06 to 28%, and up to 29% between the complex and its tephritid outgroups, B. dorsalis and C. capitata. According to the phylogenetic relationships, these members of the B. tau complex could be classified into four clades. Thus, species A and D form clades 1 and 3, respectively, while species C and I belong to clade 4. However, species B, E, and F form a distinct group, clade 2, and infested the fruits of non-cucurbit hosts. Host-plant shifts resulting in adaptive radiation and premating isolation among species might play an important role in species differentiation of the B. tau complex.
我们以橘小实蝇、梨小实蝇、地中海实蝇、冈比亚按蚊和飞蝗作为外群，比较了果实蝇复合种8个物种的线粒体细胞色素氧化酶I基因序列。对一个639 bp的可变区进行了测序。果实蝇复合种内物种间的序列差异为0.06%至28%，该复合种与其实蝇科外群橘小实蝇和地中海实蝇之间的差异高达29%。根据系统发育关系，果实蝇复合种的这些成员可分为四个分支。因此，物种A和D分别形成分支1和3，而物种C和I属于分支4。然而，物种B、E和F形成一个独特的类群，即分支2，它们侵害非葫芦科寄主的果实。导致适应性辐射和物种间交配前隔离的寄主植物转移可能在果实蝇复合种的物种分化中起重要作用。
