First University Department of Obstetrics and Gynecology, Alexandra General Hospital, University of Athens, Athens, Greece.
Reprod Biol Endocrinol. 2012 Sep 7;10:74. doi: 10.1186/1477-7827-10-74.
The purpose of the study was to determine the incidence of survivin gene expression in human granulosa cells during ovarian stimulation in Greek women with normal FSH levels, undergoing IVF or ICSI and to discover any correlation between levels of gene expression and clinical parameters, efficacy of ovulation or outcomes of assisted reproduction.
Twenty nine women underwent ovulation induction for IVF or ICSI and ET with standard GnRH analogue-recombinant FSH protocol. Infertility causes were male and tubal factor. Cumulus-mature oocyte complexes were denuded and the granulosa cells were analyzed for each patient separately using quantitative reverse transcription polymerase chain reaction analysis for survivin gene expression with internal standard the ABL gene.
The ABL and survivin mRNA were detected in granulosa cells in 93.1%. The expression levels of survivin were significantly lower in normal women (male infertility factor) compared to women with tubal infertility factor (p = 0.007). There was no additional statistically significant correlation between levels of survivin expression and estradiol levels or dosage of FSH for ovulation induction or number of dominant follicles aspirated or number of retrieved oocytes or embryo grade or clinical pregnancy rates respectively.
High levels of survivin mRNA expression in luteinized granulosa cells in cases with tubal infertility seem to protect ovaries from follicular apoptosis. A subpopulation of patients with low levels of survivin mRNA in granulosa cells might benefit with ICSI treatment to bypass possible natural barriers of sperm-oocyte interactions.
本研究旨在确定在接受体外受精或卵胞浆内单精子注射（ICSI）和胚胎移植（ET）的希腊正常促卵泡激素（FSH）水平女性中，人卵巢颗粒细胞中生存素基因表达的发生率，并发现基因表达水平与临床参数、排卵效果或辅助生殖结局之间的任何相关性。
29 名女性因男性和输卵管因素不孕接受了标准 GnRH 类似物-重组 FSH 方案的 IVF 或 ICSI 及 ET 促排卵。分别对每位患者的卵丘-成熟卵母细胞复合物进行去卵丘处理，并使用定量逆转录聚合酶链反应分析（qRT-PCR）分析生存素基因表达，内部标准为 ABL 基因。
在 93.1%的颗粒细胞中检测到 ABL 和 survivin mRNA。与输卵管不孕因素的女性相比，正常女性（男性不孕因素）的生存素表达水平显著降低（p=0.007）。生存素表达水平与雌激素水平或促排卵 FSH 剂量、抽吸的优势卵泡数、获取的卵母细胞数、胚胎分级或临床妊娠率之间无其他统计学显著相关性。
在输卵管不孕患者的黄体化颗粒细胞中高水平的 survivin mRNA 表达似乎可保护卵巢免受卵泡细胞凋亡。颗粒细胞中 survivin mRNA 水平较低的亚群患者可能受益于 ICSI 治疗，以绕过精子-卵母细胞相互作用的可能自然障碍。
