Yamashita Yoshiki, Ueda Masatsugu, Takehara Mikio, Yamashita Hikari, Suzuki Yoshihiko, Hung Yao-Ching, Terai Yoshito, Ueki Minoru
Department of Obstetrics and Gynecology, Osaka Medical College, Takatsuki, Osaka, Japan.
Fertil Steril. 2002 Oct;78(4):865-71. doi: 10.1016/s0015-0282(02)03339-3.
To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells.
Prospective study.
IVF-ET program at Osaka Medical College.
PATIENT(S): Seventeen patients with revised American Fertility Society stage IV endometriosis and 17 patients with tubal infertility and no endometriosis.
INTERVENTION(S): Granulosa cells obtained at oocyte retrieval were examined for VEGF and IL-6 gene expression.
MAIN OUTCOME MEASURE(S): Serum E(2) and P levels at hCG administration, number of oocytes, fertilization rate, high-quality embryo rate, and pregnancy rate, and expression of VEGF and IL-6 genes.
RESULT(S): Total hMG and FSH levels were statistically significantly higher in patients with endometriosis; however, the number of retrieved oocytes and the fertilization rate were lower compared with patients with tubal infertility. Serum E(2) levels and expression of VEGF in patients with tubal infertility were statistically significantly higher than those in patients with endometriosis. Interleukin-6 gene expression did not differ between the groups.
CONCLUSION(S): In severe endometriosis, lower VEGF gene expression in granulosa cells may adversely affect oocyte development and maturation.
评估子宫内膜异位症如何影响颗粒细胞中血管内皮生长因子（VEGF）和白细胞介素-6（IL-6）的表达。
前瞻性研究。
大阪医科大学体外受精-胚胎移植项目。
17例经修订的美国生育协会IV期子宫内膜异位症患者和17例输卵管性不孕且无子宫内膜异位症的患者。
对取卵时获得的颗粒细胞进行VEGF和IL-6基因表达检测。
注射人绒毛膜促性腺激素（hCG）时的血清雌二醇（E₂）和孕酮（P）水平、卵母细胞数量、受精率、优质胚胎率和妊娠率，以及VEGF和IL-6基因的表达。
子宫内膜异位症患者的总人绝经期促性腺激素（hMG）和促卵泡生成素（FSH）水平在统计学上显著更高；然而，与输卵管性不孕患者相比，取到的卵母细胞数量和受精率更低。输卵管性不孕患者的血清E₂水平和VEGF表达在统计学上显著高于子宫内膜异位症患者。两组间白细胞介素-6基因表达无差异。
在重度子宫内膜异位症中，颗粒细胞中VEGF基因表达降低可能会对卵母细胞发育和成熟产生不利影响。
