人类T细胞抗原受体δ链基因转录的调控。一个T细胞系特异性增强子元件位于Jδ3-Cδ内含子中。
Regulation of transcription of the human T cell antigen receptor delta chain gene. A T lineage-specific enhancer element is located in the J delta 3-C delta intron.
作者信息
Bories J C, Loiseau P, d'Auriol L, Gontier C, Bensussan A, Degos L, Sigaux F
机构信息
Laboratoire d'Hématologie Moléculaire, Hôpital St. Louis, Paris, France.
出版信息
J Exp Med. 1990 Jan 1;171(1):75-83. doi: 10.1084/jem.171.1.75.
Abstract
We have defined transcriptional enhancing sequences inside the TCR-delta gene locus, using transient transfections with constructs containing DNA fragments cloned upstream to a reporter gene fused to a heterologous promoter. A 14-kb DNA region extending from the J delta 3 segment to 6 kb 3' to C delta was analyzed. We show the presence of positive regulatory sequences inside the J delta 3-C delta intron and have localized these sequences to two DNA fragments of approximately 300 and 258 bp. Analysis of cell specificity of the activation of such sequences demonstrates a T cell pattern for one of the two fragments. The nucleotide sequence of the T cell-specific element shows motifs sharing homology with previously described core enhancers.
摘要
我们利用瞬时转染技术，通过构建包含克隆至与异源启动子融合的报告基因上游的DNA片段的载体，在TCR-δ基因座内定义了转录增强序列。分析了从Jδ3区段延伸至Cδ下游6 kb的14 kb DNA区域。我们发现Jδ3-Cδ内含子内存在正调控序列，并将这些序列定位到两个大小约为300 bp和258 bp的DNA片段。对这些序列激活的细胞特异性分析表明，两个片段之一具有T细胞模式。T细胞特异性元件的核苷酸序列显示出与先前描述的核心增强子具有同源性的基序。
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