Centro de Investigaciones Quimicas, Universidad Autonoma el Estado de Hidalgo, Carr. Pachuca-Tulancingo km 4.5, C.P. 42076, Pachuca, Hidalgo, Mexico.
Biosens Bioelectron. 2013 Mar 15;41:244-8. doi: 10.1016/j.bios.2012.08.033. Epub 2012 Aug 23.
An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)(2)FeCl(4). The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0mM glucose solution was 1.0%.The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed.
介绍了一种安培流动系统，用于在酶促反应后，通过固定在硅胶覆盖的磁铁上的葡萄糖氧化酶将血清样本中的葡萄糖转化为简体中文。固体通过改良的筛网印刷电极[Fe（tris（3,5-二甲基-1-吡唑基）硼酸）（2）]+[FeCl（4）]（-）在流动注射系统之前被磁性保留，用于安培检测。系统中的变量，如流速、酶浓度、注射体积和反应盘管长度，使用田口参数设计进行了评估。在最佳条件下，葡萄糖样品的校准曲线在 0.24 和 6.00 mM 之间呈线性，检测限为 0.08 mM。对于 4.0mM 葡萄糖溶液，重复性为 1.0%。该方法通过将获得的结果与酶分光光度法提供的结果进行比较进行了验证；未观察到显著差异。
