透明质酸功能化介孔硅纳米粒子用于癌细胞的高效光动力治疗。
Hyaluronic acid-functionalized mesoporous silica nanoparticles for efficient photodynamic therapy of cancer cells.
机构信息
Institut des Biomolécules Max Mousseron UMR 5247 CNRS, Université Montpellier 1, Université Montpellier 2 - Faculté de Pharmacie, 15 Avenue Charles Flahault, 34093 Montpellier Cedex 05, France.
出版信息
Photodiagnosis Photodyn Ther. 2012 Sep;9(3):256-60. doi: 10.1016/j.pdpdt.2011.12.010. Epub 2012 Jan 31.
Mesoporous silica nanoparticles (MSN) for photodynamic therapy (PDT) were coated with poly-(L-lysine) and hyaluronic acid (HA) by using the layer-by-layer method. HA is able to target cancer cells over-expressing the corresponding CD44 receptor. MSN functionalized with HA (MSN-HA) were more efficient than MSN without the targeting moiety when PDT was performed at low fluence (14 Jcm(-2)) and low dosage of MSN (20 μgmL(-1)) on HCT 116 colorectal cancer cells, known to over-express the CD44 receptor. Incubation of HCT-116 cancer cells with an excess of HA impaired the PDT effect with MSN-HA thus demonstrating that an active endocytosis mechanism was involved in the uptake of MSN-HA by these cells.
介孔硅纳米粒子（MSN）通过层层自组装法被聚-L-赖氨酸和透明质酸（HA）包被。HA 能够靶向过表达相应 CD44 受体的癌细胞。在低剂量（20μgmL（-1））和低光强（14 Jcm（-2））条件下进行光动力治疗（PDT）时，与没有靶向基团的 MSN 相比，用 HA 功能化的 MSN（MSN-HA）对 HCT116 结直肠癌细胞（已知过表达 CD44 受体）的 PDT 效果更好。用过量的 HA 孵育 HCT-116 癌细胞会损害 MSN-HA 的 PDT 效果，这表明主动内吞机制参与了这些细胞对 MSN-HA 的摄取。
Zotero 插件