FBK, Via Sommarive 18, Povo 38123 Trento, Italy.
Int J Pharm. 2012 Nov 15;438(1-2):45-52. doi: 10.1016/j.ijpharm.2012.08.057. Epub 2012 Sep 7.
Gold nanoparticles functionalized with doxorubicin and stabilized with multilayers of degradable polyelectrolyte were allowed to age in aqueous medium in vitro in order to show the possibility of drug release in cellular environment. The chemico-physical characteristics of the nanoparticles are reported. The observed release of doxorubicin (DOX) was pH-dependent, and it increased in acidic environment. Cell uptake of nanoparticles and drug release were monitored by laser scanning confocal microscopy. Data showed that drug-bearing nanoparticles delivered DOX into the nuclei of A549 cells, leading to pronounced cytotoxic effects to this lung tumor cells. Our results suggest that gold nanoparticles conjugated with doxorubicin could be used as a pH-triggered drug releasing carrier for tumor drug delivery.
用阿霉素功能化并通过可降解聚电解质多层稳定的金纳米粒子在体外水介质中老化，以显示在细胞环境中释放药物的可能性。报道了纳米粒子的理化特性。观察到阿霉素（DOX）的释放与 pH 值有关，并在酸性环境中增加。通过激光扫描共聚焦显微镜监测纳米粒子的细胞摄取和药物释放。数据表明，载药纳米粒子将 DOX 递送到 A549 细胞的核内，导致对这种肺肿瘤细胞产生明显的细胞毒性作用。我们的结果表明，与阿霉素偶联的金纳米粒子可用作 pH 触发的药物释放载体，用于肿瘤药物递送。
