Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China.
Int J Biol Macromol. 2012 Dec;51(5):1134-9. doi: 10.1016/j.ijbiomac.2012.08.037. Epub 2012 Sep 7.
Limonium sinense (Girard) Kuntze is a traditional Chinese folk medicine used for the treatment of fever, hemorrhage, hepatitis and other disorders. The study focused on the antitumor and immunomodulatory activities of L. sinense polysaccharides (LSP) which was obtained from the root of the plant. The antitumor effects of only LSP and LSP in combination with 5-fluorouracil (5-FU) were both evaluated with Heps-bearing tumor mice models. In addition, the macrophage phagocytosis assay, splenocyte proliferation and cytokines production tests were used to assess the immunomodulatory activities of LSP. The results revealed that the LSP (at the dose of 200 and 400 mg/kg) had an obvious inhibition on the growth of transplanted mouse tumor. It also exhibited a significant synergistic effect of antitumor activity when combined with 5-FU (p<0.05). Furthermore, the LSP (at the dose of 100 and 200 mg/kg) remarkably improved macrophage phagocytosis function in immune suppressed mice. In addition, LSP (at the dose of 50-200 μg/ml) showed significant synergistic effects on ConA-stimulated proliferation and IFN-γ and IL-2 production of splenocyte in vitro (p<0.05). These findings suggest that LSP had clear antitumor activity which might be related to its regulation of immune function in mice.
中华补血草（Girard）Kuntze 是一种传统的中药，用于治疗发热、出血、肝炎等疾病。本研究集中于中华补血草多糖（LSP）的抗肿瘤和免疫调节活性，该多糖是从植物根部获得的。仅用 LSP 和 LSP 与 5-氟尿嘧啶（5-FU）联合治疗荷瘤小鼠模型，评估了 LSP 的抗肿瘤作用。此外，还进行了巨噬细胞吞噬试验、脾细胞增殖和细胞因子产生试验，以评估 LSP 的免疫调节活性。结果表明，LSP（剂量为 200 和 400 mg/kg）对移植小鼠肿瘤的生长有明显的抑制作用，与 5-FU 联合使用时具有显著的协同抗肿瘤活性（p<0.05）。此外，LSP（剂量为 100 和 200 mg/kg）显著改善了免疫抑制小鼠的巨噬细胞吞噬功能。此外，LSP（剂量为 50-200 μg/ml）在体外对 ConA 刺激的脾细胞增殖和 IFN-γ和 IL-2 的产生具有显著的协同作用（p<0.05）。这些发现表明，LSP 具有明显的抗肿瘤活性，这可能与其在小鼠体内调节免疫功能有关。
