Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
Environ Toxicol Pharmacol. 2012 Nov;34(3):949-58. doi: 10.1016/j.etap.2012.07.014. Epub 2012 Aug 4.
Colorectal cancer is one of the leading causes of cancer related deaths in Western countries and is becoming increasingly common in Asia. Rosmarinic acid (RA), one of the major components of polyphenol possesses attractive remedial features. The purpose of this study is to investigate the possible chemopreventive mechanism of action of RA against 1,2-dimethylhydrazine (DMH) induced rat colon carcinogenesis by evaluating the circulatory antioxidant status and colonic bacterial enzymes activities. Additionally, we analyzed the aberrant crypt foci (ACF) formation and multiplicity in the colon of experimental groups. Wistar male rats were divided into six groups. Group 1 was control rats, group 2 rats received RA (10 mg/kg b.w., p.o. everyday), rats in groups 3-6 received DMH (20 mg/kg b.w., s.c.) for the first 4 weeks. In addition to DMH, groups 4-6 received 2.5, 5, and 10 mg/kg b.w. RA respectively. The results revealed that supplementation with RA significantly reduced the formation of ACF and ACF multiplicity in DMH treated rats. Moreover RA supplementation prevented the alterations in circulatory antioxidant enzymes and colonic bacterial enzymes activities. Overall, our results showed that all three doses of RA inhibited carcinogenesis, though the effect of the intermediary dose of 5 mg/kg b.w. was more pronounced.
结直肠癌是西方国家癌症相关死亡的主要原因之一，在亚洲也越来越常见。迷迭香酸（RA）是多酚的主要成分之一，具有吸引人的治疗特性。本研究旨在通过评估循环抗氧化状态和结肠细菌酶活性，研究 RA 对 1,2-二甲基肼（DMH）诱导的大鼠结肠癌发生的可能化学预防作用机制。此外，我们还分析了实验组结肠中异常隐窝病灶（ACF）的形成和多发性。雄性 Wistar 大鼠分为六组。第 1 组为对照组，第 2 组大鼠给予 RA（10 mg/kg bw，po，每天），第 3-6 组大鼠给予 DMH（20 mg/kg bw，sc），第 4-6 组大鼠除给予 DMH 外，还分别给予 2.5、5 和 10 mg/kg bw RA。结果表明，RA 补充剂可显著减少 DMH 处理大鼠 ACF 的形成和多发性。此外，RA 补充剂可预防循环抗氧化酶和结肠细菌酶活性的改变。总的来说，我们的结果表明，所有三种剂量的 RA 均抑制了致癌作用，尽管中间剂量 5 mg/kg bw 的作用更为明显。
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