Kim Da-Hye, Kim Sang Jun, Yu Kang-Yeol, Jeong Seung-Il, Kim Seon-Young
Jeonju AgroBio-Materials Institute, 111-27, Wonjangdong-gil, Deokjin-gu, Jeonju, Jeonbuk 54810, Korea.
Nutr Res Pract. 2018 Feb;12(1):20-28. doi: 10.4162/nrp.2018.12.1.20. Epub 2018 Jan 12.
BACKGROUND/OBJECTIVES: (L.) Britton . (PF) sprout is a plant of the labiate family. We have previously reported the protective effects of PF sprout extract on cytokine-induced β-cell damage. However, the mechanism of action of the PF sprout extract in type 2 diabetes (T2DM) has not been investigated. The present study was designed to study the effects of PF sprout extract and signaling mechanisms in the T2DM mice model using C57BL/KsJ-db/db (db/db) mice.
MATERIALS/METHODS: Male db/db mice were orally administered PF sprout extract (100, 300, and 1,000 mg/kg of body weight) or rosiglitazone (RGZ, positive drug, 1 mg/kg of body weight) for 4 weeks. Signaling mechanisms were analyzed using liver tissues and HepG2 cells.
The PF sprout extract (300 and 1,000 mg/kg) significantly reduced the fasting blood glucose, serum insulin, triglyceride and total cholesterol levels in db/db mice. PF sprout extract also significantly improved glucose intolerance and insulin sensitivity, decreased hepatic gluconeogenic protein expression, and ameliorated histological alterations of the pancreas and liver. Levels of phosphorylated AMP-activated protein kinase (AMPK) protein expression also increased in the liver after treatment with the extract. In addition, an increase in the phosphorylation of AMPK and decrease in the phosphoenolpyruvate carboxykinase and glucose 6-phosphatase proteins in HepG2 cells were also observed.
Our results sugges that PF sprout displays beneficial effects in the prevention and treatment of type 2 diabetes via modulation of the AMPK pathway and inhibition of gluconeogenesis in the liver.
背景/目的：假荆芥（L.）布里顿. （PF）芽是唇形科植物。我们之前报道过PF芽提取物对细胞因子诱导的β细胞损伤具有保护作用。然而，PF芽提取物在2型糖尿病（T2DM）中的作用机制尚未得到研究。本研究旨在使用C57BL/KsJ-db/db（db/db）小鼠研究PF芽提取物在T2DM小鼠模型中的作用及信号机制。
材料/方法：雄性db/db小鼠口服给予PF芽提取物（100、300和1000 mg/kg体重）或罗格列酮（RGZ，阳性药物，1 mg/kg体重），持续4周。使用肝脏组织和HepG2细胞分析信号机制。
PF芽提取物（300和1000 mg/kg）显著降低了db/db小鼠的空腹血糖、血清胰岛素、甘油三酯和总胆固醇水平。PF芽提取物还显著改善了葡萄糖耐量和胰岛素敏感性，降低了肝脏糖异生蛋白表达，并改善了胰腺和肝脏的组织学改变。提取物处理后，肝脏中磷酸化的AMP激活蛋白激酶（AMPK）蛋白表达水平也有所增加。此外，在HepG2细胞中也观察到AMPK磷酸化增加以及磷酸烯醇丙酮酸羧激酶和葡萄糖6磷酸酶蛋白减少。
我们的结果表明，PF芽通过调节AMPK途径和抑制肝脏糖异生在2型糖尿病的预防和治疗中显示出有益作用。
