Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Inflamm Res. 2013 Jan;62(1):61-7. doi: 10.1007/s00011-012-0551-6. Epub 2012 Sep 9.
Endotoxemia is a major cause of mortality in large animals and there are several therapeutic regimens for the treatment of endotoxemia. Recent studies have suggested the anti-inflammatory effects of insulin in endotoxemic human and laboratory animal models but to the best of our knowledge there is no report on the possible therapeutic effect of insulin in large animal endotoxemia.
This experiment was conducted to evaluate the anti-inflammatory effects of insulin regular compared with flunixin meglumine on the treatment of endotoxemia in sheep.
Lipopolysaccharide from Escherichia coli was administered intravenously to ewes. Anti-inflammatory effects of flunixin meglumine (at 2.2 mg/kg) and insulin regular (at 1.5 and 3 IU/kg) were evaluated by determination of serum concentrations of acute phase proteins, inflammatory cytokines and oxidative stress biomarkers.
Insulin regular at 3 IU/kg controlled the acute phase response following endotoxemia induction. The anti-inflammatory potency of insulin regular at 3 IU/kg was significantly higher than at 1.5 IU/kg and of flunixin meglumine at 2.2 mg/kg (P < 0.05).
Insulin regular induces its anti-inflammatory effects in a dose-dependent manner. Intravenous use of insulin regular can be a potential new therapeutic regimen for endotoxemia in large animal medicine.
内毒素血症是大型动物死亡的主要原因，有几种治疗内毒素血症的治疗方案。最近的研究表明胰岛素在人类和实验动物模型中的抗炎作用，但据我们所知，尚无关于胰岛素对内毒素血症的大型动物可能治疗效果的报道。
本实验旨在评估胰岛素常规与氟尼辛葡甲胺对绵羊内毒素血症的抗炎作用。
通过静脉注射大肠杆菌脂多糖，来给母羊注射。通过测定急性期蛋白、炎症细胞因子和氧化应激生物标志物来评估氟尼辛葡甲胺（2.2mg/kg）和胰岛素常规（1.5 和 3IU/kg）的抗炎效果。
胰岛素常规 3IU/kg 对内毒素血症诱导后的急性期反应有控制作用。胰岛素常规 3IU/kg 的抗炎效力明显高于 1.5IU/kg 和氟尼辛葡甲胺 2.2mg/kg（P<0.05）。
胰岛素常规以剂量依赖的方式发挥其抗炎作用。静脉使用胰岛素常规可能成为大型动物医学中治疗内毒素血症的一种新的潜在治疗方案。
