Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, China.
J Surg Oncol. 2013 Apr;107(5):555-9. doi: 10.1002/jso.23264. Epub 2012 Sep 7.
Long intergenic non-coding RNAs (lincRNAs) are a class of non-coding RNAs that regulate gene expression via chromatin reprogramming. Taurine Up-regulated Gene 1 (TUG1) is a lincRNA that is associated with chromatin-modifying complexes and plays roles in gene regulation. In this study, we determined the expression patterns of TUG1 and the cell proliferation inhibition and apoptosis induced by silencing TUG1 in urothelial carcinoma of the bladder.
The expression levels of TUG1 were determined using Real-Time qPCR in a total of 44 patients with bladder urothelial carcinomas. Bladder urothelial carcinoma T24 and 5637 cells were transfected with TUG1 siRNA or negative control siRNA. Cell proliferation was evaluated using MTT assay. Apoptosis was determined using ELISA assay.
TUG1 was up-regulated in bladder urothelial carcinoma compared to paired normal urothelium. High TUG1 expression levels were associated with high grade and stage carcinomas. Cell proliferation inhibition and apoptosis induction were observed in TUG1 siRNA-transfected bladder urothelial carcinoma T24 and 5637 cells.
Our data suggest that lincRNA TUG1 is emerging as a novel player in the disease state of bladder urothelial carcinoma. TUG1 may have potential roles as a biomarker and/or a therapeutic target in bladder urothelial carcinoma.
长链非编码 RNA（lncRNA）是一类通过染色质重编程调节基因表达的非编码 RNA。牛磺酸上调基因 1（TUG1）是一种 lincRNA，与染色质修饰复合物相关，在基因调控中发挥作用。在本研究中，我们确定了 TUG1 的表达模式以及沉默 TUG1 对膀胱尿路上皮癌细胞增殖抑制和凋亡的影响。
采用 Real-Time qPCR 检测 44 例膀胱尿路上皮癌患者的 TUG1 表达水平。用 TUG1 siRNA 或阴性对照 siRNA 转染膀胱尿路上皮癌细胞株 T24 和 5637。采用 MTT 法检测细胞增殖，ELISA 法检测细胞凋亡。
与配对的正常尿路上皮相比，膀胱尿路上皮癌中 TUG1 表达上调。高 TUG1 表达水平与高级别和晚期膀胱癌相关。在 TUG1 siRNA 转染的膀胱尿路上皮癌细胞株 T24 和 5637 中观察到细胞增殖抑制和凋亡诱导。
我们的数据表明，lncRNA TUG1 是膀胱癌疾病状态中的一个新的调控因子。TUG1 可能作为膀胱癌的生物标志物和/或治疗靶点具有潜在作用。
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