Department of Nutrition, Biomedical Science Laboratory, China Medical University, Taichung, Taiwan.
Mol Nutr Food Res. 2012 Oct;56(10):1520-31. doi: 10.1002/mnfr.201200098. Epub 2012 Sep 7.
Our previous report showed that concomitant supplementation of lycopene and eicosa-pentaenoic acid synergistically inhibited the proliferation of human colon cancer HT-29 cells in vitro.
To validate our findings, the present study investigated whether consumption of lycopene and fish oil would help prevent tumor growth and progression in a mouse xenograft model of colon cancer. The inhibitory effects of lycopene and fish oil on tumor growth were verified by western blotting analysis, bioluminescent imaging, immunohistochemical (IHC) staining and ELISA. The results demonstrated that lycopene and fish oil synergistically inhibited the growth of colon cancer in tumor-bearing mice. The bioluminescent imaging, histopathological and IHC staining results indicated that lycopene and fish oil effectively suppressed tumor growth and progression of colon cancer in vivo. The chemopreventive effects of lycopene and fish oil were associated with augmented expression of the cell cycle inhibitors such as p21(CIP1/WAF1) and p27(Kip1) , and suppression of proliferating cell nuclear antigen, β-catenin, cyclin D1 and c-Myc proteins. Furthermore, lycopene and fish oil inhibited tumor progression through suppression of MMP-7, MMP-9, COX-2 and PGE2.
These results show that lycopene and fish oil act synergistically as chemopreventive agents against tumor growth and progression in a mouse xenograft model of colon cancer.
我们之前的报告表明，番茄红素和二十碳五烯酸的同时补充协同抑制了人结肠癌 HT-29 细胞的体外增殖。
为了验证我们的发现，本研究调查了番茄红素和鱼油的摄入是否有助于预防结肠癌小鼠异种移植模型中的肿瘤生长和进展。通过 Western 印迹分析、生物发光成像、免疫组织化学（IHC）染色和 ELISA 验证了番茄红素和鱼油对肿瘤生长的抑制作用。结果表明，番茄红素和鱼油协同抑制了荷瘤小鼠结肠癌的生长。生物发光成像、组织病理学和 IHC 染色结果表明，番茄红素和鱼油有效抑制了结肠癌在体内的生长和进展。番茄红素和鱼油的化学预防作用与细胞周期抑制剂如 p21(CIP1/WAF1)和 p27(Kip1)的表达增强有关，并抑制了增殖细胞核抗原、β-连环蛋白、细胞周期蛋白 D1 和 c-Myc 蛋白的表达。此外，番茄红素和鱼油通过抑制 MMP-7、MMP-9、COX-2 和 PGE2 抑制肿瘤进展。
这些结果表明，番茄红素和鱼油在结肠癌小鼠异种移植模型中作为化学预防剂协同作用，抑制肿瘤生长和进展。
