Biomedical Science Laboratory, Department of Nutrition, China Medical University, Taichung, Taiwan, Republic of China.
J Agric Food Chem. 2011 Aug 24;59(16):9011-21. doi: 10.1021/jf2017644. Epub 2011 Jul 25.
A previous study indicated that lycopene could significantly inhibit the proliferation of human colon cancer cells in vitro. However, the in vivo anticancer effects of lycopene against colon cancer have not been demonstrated yet. Therefore, this study investigated whether consumption of lycopene could prevent the growth and progression of colorectal tumor in a mouse xenograft model. Bioluminescence imaging, histopathological, immunofluorescence (IFC), and immunohistochemical (IHC) staining results indicated that lycopene could effectively suppress the growth and progression of colon cancer in tumor-bearing mice. The results demonstrated that lycopene significantly suppressed the nuclear expression of PCNA and β-catenin proteins in tumor tissues. Consumption of lycopene could also augment the E-cadherin adherent molecule and nuclear levels of cell cycle inhibitor p21(CIP1/WAF1) protein. The chemopreventive effects of lycopene were associated with suppression of COX-2, PGE(2), and phosphorylated ERK1/2 proteins. Furthermore, the inhibitory effects of lycopene were inversely correlated with the plasma levels of matrix metalloproteinase 9 (MMP-9) in tumor-bearing mice. These results suggested that lycopene could act as a chemopreventive agent against the growth and progression of colorectal cancer in a mouse xenograft model.
先前的研究表明，番茄红素可显著抑制人结肠癌细胞在体外的增殖。然而，番茄红素对结肠癌的体内抗癌作用尚未得到证实。因此，本研究旨在探讨番茄红素的摄入是否可以预防结直肠肿瘤在荷瘤小鼠模型中的生长和进展。生物发光成像、组织病理学、免疫荧光（IFC）和免疫组织化学（IHC）染色结果表明，番茄红素可有效抑制荷瘤小鼠结肠癌的生长和进展。结果表明，番茄红素可显著抑制肿瘤组织中 PCNA 和 β-catenin 蛋白的核表达。番茄红素的摄入还可以增加细胞周期抑制剂 p21（CIP1/WAF1）蛋白的 E-钙黏附分子和核水平。番茄红素的化学预防作用与 COX-2、PGE（2）和磷酸化 ERK1/2 蛋白的抑制有关。此外，番茄红素的抑制作用与荷瘤小鼠血浆中基质金属蛋白酶 9（MMP-9）的水平呈负相关。这些结果表明，番茄红素可作为一种化学预防剂，抑制结直肠肿瘤在荷瘤小鼠模型中的生长和进展。
