Tonemura Y, Kamata T, Ooyama S, Sugiyama K, Kimura H, Kosaka T, Yamaguchi A, Miyazaki I
Department of Surgery II, School of Medicine, Kanazawa University, Japan.
J Surg Oncol. 1990 Jan;43(1):3-7. doi: 10.1002/jso.2930430103.
The prognostic significance of proliferative activity in 167 gastric carcinomas was studied by means of an in vitro labeling method with bromodeoxyuridine (BrdU), using specimens obtained by endoscopic biopsy, and the results were correlated with conventional prognostic factors. The BrdU labeling indicates (BrdU LI) varied from 2.1 to 45%. When the in vitro BrdU LI were plotted against in vivo BrdU LI, a significant correlation was found between in vitro and in vivo BrdU LI (P less than .01). These results show that the in vitro BrdU LI of specimens obtained by endoscopic biopsy indicate accurate proliferative activities of gastric cancers. The BrdU LI were closely correlated with nodal status, vessel invasion, and tumor size. With regard to the correlation of BrdU LI and prognosis, patients whose tumors had a high BrdU LI (BrdU greater than LI less than 10%). When the BrdU LI and all the pathologic findings were entered simultaneously into the regression model, nodal status, tumor size, serosal involvement, and BrdU LI emerged as independent prognostic parameters. The present studies demonstrated the usefulness of the in vitro BrdU-labeling method to estimate the malignancy of gastric cancer preoperatively, and this method may be useful in designing the operative procedure and multimodal therapy for individual patients.
采用溴脱氧尿苷（BrdU）体外标记法，利用内镜活检获取的标本，对167例胃癌的增殖活性的预后意义进行了研究，并将结果与传统预后因素进行了关联分析。BrdU标记指数（BrdU LI）在2.1%至45%之间变化。当将体外BrdU LI与体内BrdU LI进行绘图时，发现体外和体内BrdU LI之间存在显著相关性（P小于0.01）。这些结果表明，通过内镜活检获取的标本的体外BrdU LI能够准确反映胃癌的增殖活性。BrdU LI与淋巴结状态、血管侵犯和肿瘤大小密切相关。关于BrdU LI与预后的相关性，肿瘤BrdU LI高的患者（BrdU大于LI小于10%）。当将BrdU LI和所有病理结果同时纳入回归模型时，淋巴结状态、肿瘤大小、浆膜受累和BrdU LI成为独立的预后参数。本研究证明了体外BrdU标记法在术前评估胃癌恶性程度方面的有用性，该方法可能有助于为个体患者设计手术方案和多模式治疗。
