University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1793-1799. doi: 10.1099/ijs.0.043679-0. Epub 2012 Sep 7.
A Gram-staining-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated DPS-8(T), was isolated from coastal sediment of Geoje island in the South Sea, South Korea, and subjected to a polyphasic study. Strain DPS-8(T) grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DPS-8(T) joined the clade comprising the type strains of Winogradskyella species with a high bootstrap resampling value of 93.5 %. Phylogenetic trees constructed using maximum-likelihood and maximum-parsimony algorithms revealed that strain DPS-8(T) belonged to the genus Winogradskyella. Strain DPS-8(T) exhibited 94.1-96.5 % 16S rRNA gene sequence similarity to the type strains of species of the genus Winogradskyella. Strain DPS-8(T) contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 1ω7c and/or iso-C15 : 0 2-OH as the major fatty acids. The major polar lipids of strain DPS-8(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain DPS-8(T) was 34.7 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain DPS-8(T) is separate from recognized species of the genus Winogradskyella. On the basis of the data presented, strain DPS-8(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella litorisediminis sp. nov. is proposed. The type strain is DPS-8(T) ( = KCTC 32110(T) = CCUG 62215(T)).
一株革兰氏阴性、无鞭毛、不滑行、需氧、杆状细菌，被命名为 DPS-8(T)，从韩国南海巨济岛的沿海沉积物中分离得到，并进行了多相研究。菌株 DPS-8(T) 在 30°C、pH7.0-7.5 和 2%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列的邻接法系统发育树显示，菌株 DPS-8(T) 与 Winogradskyella 属的模式菌株聚在一起，自举值为 93.5%。使用最大似然法和最大简约法构建的系统发育树表明，菌株 DPS-8(T) 属于 Winogradskyella 属。菌株 DPS-8(T) 与 Winogradskyella 属的模式菌株的 16S rRNA 基因序列相似性为 94.1-96.5%。菌株 DPS-8(T) 含有 MK-6 作为主要的menaquinone，以及 iso-C15:1 G、iso-C15:0、iso-C17:0 3-OH 和 C16:1ω7c 和/或 iso-C15:0 2-OH 作为主要脂肪酸。菌株 DPS-8(T) 的主要极性脂为磷脂酰乙醇胺和两种未鉴定的脂类。菌株 DPS-8(T) 的 DNA G+C 含量为 34.7mol%。表型差异特征以及其系统发育上的独特性表明，菌株 DPS-8(T) 与公认的 Winogradskyella 属的种不同。基于所提出的数据，菌株 DPS-8(T) 被认为是代表 Winogradskyella 属的一个新种，命名为 Winogradskyella litorisediminis sp. nov.。模式菌株为 DPS-8(T)（ = KCTC 32110(T) = CCUG 62215(T)）。
