Cao Pan, Li Wen-Wu, Morris Andrew R, Horrocks Paul D, Yuan Cheng-Qing, Yang Ying
School of Energy and Power Engineering, Wuhan University of Technology, Wuhan 430063, People's Republic of China.
Institute for Science and Technology in Medicine, Keele University, Stoke-on-Trent ST4 7QB, UK.
R Soc Open Sci. 2018 Mar 7;5(3):172165. doi: 10.1098/rsos.172165. eCollection 2018 Mar.
Biofilm formation on surfaces is an important research topic in ship tribology and medical implants. In this study, dopamine and two types of synthetic peptides were designed and attached to 304 stainless steel surfaces, aiming to inhibit the formation of biofilms. A combinatory surface modification procedure was applied in which dopamine was used as a coupling agent, allowing a strong binding ability with the two peptides. X-ray photoelectron spectroscopy (XPS), elemental analysis, contact angle measurement and surface roughness test were used to evaluate the efficiency of the peptide modification. An antibiofilm assay against was conducted to validate the antibiofilm capacity of the peptide-modified stainless steel samples. XPS analysis confirmed that the optimal dopamine concentration was 40 µg ml in the coupling reaction. Element analysis showed that dopamine and the peptides had bound to the steel surfaces. The robustness assay of the modified surface demonstrated that most peptide molecules had bound on the surface of the stainless steel firmly. The contact angle of the modified surfaces was significantly changed. Modified steel samples exhibited improved antibiofilm properties in comparison to untreated and dopamine-only counterpart, with the peptide 1 modification displaying the best antibiofilm effect. The modified surfaces showed antibacterial capacity. The antibiofilm capacity of the modified surfaces was also surface topography sensitive. The steel sample surfaces polished with 600# sandpaper exhibited stronger antibiofilm capacity than those polished with other types of sandpapers after peptide modification. These findings present valuable information for future antifouling material research.
表面生物膜的形成是船舶摩擦学和医用植入物领域的一个重要研究课题。在本研究中，设计了多巴胺和两种合成肽并将其附着于304不锈钢表面，旨在抑制生物膜的形成。采用了一种组合表面改性程序，其中多巴胺用作偶联剂，使其与两种肽具有很强的结合能力。利用X射线光电子能谱（XPS）、元素分析、接触角测量和表面粗糙度测试来评估肽改性的效率。针对[此处原文缺失具体对象]进行了抗生物膜试验，以验证肽改性不锈钢样品的抗生物膜能力。XPS分析证实，偶联反应中多巴胺的最佳浓度为40μg/ml。元素分析表明多巴胺和肽已与钢表面结合。改性表面的稳定性试验表明，大多数肽分子已牢固地结合在不锈钢表面。改性表面的接触角发生了显著变化。与未处理的和仅用多巴胺处理的对应物相比，改性钢样品表现出更好的抗生物膜性能，其中肽1改性表现出最佳的抗生物膜效果。改性表面显示出抗菌能力。改性表面的抗生物膜能力也对表面形貌敏感。肽改性后，用600#砂纸抛光的钢样品表面比用其他类型砂纸抛光的样品表现出更强的抗生物膜能力。这些发现为未来防污材料研究提供了有价值的信息。
