Department of Food Technology, Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Molecules. 2012 Sep 10;17(9):10875-92. doi: 10.3390/molecules170910875.
Natural biopolymers from plant sources contain many impurities (e.g., fat, protein, fiber, natural pigment and endogenous enzymes), therefore, an efficient purification process is recommended to minimize these impurities and consequently improve the functional properties of the biopolymer. The main objective of the present study was to investigate the effect of different purification techniques on the yield, protein content, solubility, water- and oil-holding capacity of a heteropolysaccharide-protein biopolymer obtained from durian seed. Four different purification methods using different chemicals and solvents (i.e., A (isopropanol and ethanol), B (isopropanol and acetone), C (saturated barium hydroxide), and D (Fehling solution)] to liberate the purified biopolymer from its crude form were compared. In most cases, the purification process significantly (p < 0.05) improved the physicochemical properties of heteropolysaccharide-protein biopolymer from durian fruit seed. The present work showed that the precipitation using isopropanol and acetone (Method B) resulted in the highest purification yield among all the tested purification techniques. The precipitation using saturated barium hydroxide (Method C) led to induce the highest solubility and relatively high capacity of water absorption. The current study reveals that the precipitation using Fehling solution (Method D) most efficiently eliminates the protein fraction, thus providing more pure biopolymer suitable for biological applications.
从植物来源的天然生物聚合物含有许多杂质（例如，脂肪、蛋白质、纤维、天然色素和内源性酶），因此，建议采用有效的纯化工艺来最大限度地减少这些杂质，从而提高生物聚合物的功能特性。本研究的主要目的是研究不同纯化技术对榴莲籽中获得的杂多糖-蛋白质生物聚合物的得率、蛋白质含量、溶解度、水和油保持能力的影响。使用不同的化学品和溶剂（即 A（异丙醇和乙醇）、B（异丙醇和丙酮）、C（饱和氢氧化钡）和 D（费林溶液））的四种不同的纯化方法来从粗提物中分离出纯化的生物聚合物，从而比较了四种不同的纯化方法。在大多数情况下，纯化过程显著（p < 0.05）改善了榴莲果实种子杂多糖-蛋白质生物聚合物的物理化学性质。目前的工作表明，使用异丙醇和丙酮（方法 B）沉淀可得到所有测试的纯化技术中最高的纯化收率。使用饱和氢氧化钡（方法 C）沉淀可诱导最高的溶解度和相对较高的吸水能力。本研究表明，使用费林溶液（方法 D）沉淀最有效地去除蛋白质部分，从而提供更适合生物应用的更纯的生物聚合物。
