Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, FIN-20520 Turku, Finland.
Nat Rev Genet. 2012 Oct;13(10):732-44. doi: 10.1038/nrg3271. Epub 2012 Sep 11.
Studies using high-resolution genome-wide approaches have recently reported that genomic and epigenomic alterations frequently accumulate in human pluripotent cells. Detailed characterization of these changes is crucial for understanding the impact of these alterations on self-renewal and proliferation, and particularly on the developmental and malignant potential of the cells. Such knowledge is required for the optimized and safe use of pluripotent cells for therapeutic purposes, such as regenerative cellular therapies using differentiated derivatives of pluripotent cells.In this Review, we summarize the current knowledge of the genomic and epigenomic stability of pluripotent human cells and the implications for stem cell research.
最近，使用高分辨率全基因组方法的研究报告称，基因组和表观基因组改变经常在人类多能细胞中积累。详细描述这些变化对于理解这些改变对自我更新和增殖的影响至关重要，特别是对细胞的发育和恶性潜能的影响。为了优化和安全地将多能细胞用于治疗目的，例如使用多能细胞的分化衍生物进行再生细胞治疗，这种知识是必需的。在这篇综述中，我们总结了多能人类细胞的基因组和表观基因组稳定性的现有知识及其对干细胞研究的意义。
