Laboratoire Nanobiophysique ESPCI ParisTech, CNRS UMR Gulliver 7083 10 rue Vauquelin, 75005 Paris, France.
Sci Rep. 2014 Feb 26;4:4194. doi: 10.1038/srep04194.
We describe an approach to substituting a fluorescence microarray with a surface made of an arrangement of electrolyte-gated field effect transistors. This was achieved using a dedicated blocking of non-specific interactions and comparing threshold voltage shifts of transistors exhibiting probe molecules of different base sequence. We apply the approach to detection of the 35delG mutation, which is related to non-syndromic deafness and is one of the most frequent mutations in humans. The process involves barcode sequences that are generated by Tas-PCR, a newly developed replication reaction using polymerase blocking. The barcodes are recognized by hybridization to surface attached probes and are directly detected by the semiconductor device.
我们描述了一种用由电解质门控场效应晶体管排列组成的表面替代荧光微阵列的方法。这是通过专门阻止非特异性相互作用并比较具有不同碱基序列的探针分子的晶体管的阈值电压变化来实现的。我们将该方法应用于检测与非综合征性耳聋相关的 35delG 突变，这是人类最常见的突变之一。该过程涉及 Tas-PCR 生成的条形码序列，Tas-PCR 是一种使用聚合酶阻断的新开发的复制反应。条形码通过与表面附着的探针杂交被识别，并被半导体器件直接检测。
