European Molecular Biology Laboratory and Unit of Virus Host Cell Interactions, CNRS-EMBL-UJF UMR 5322, 6 rue Jules Horowitz, F-38042 Grenoble Cedex 9, France.
Expert Rev Proteomics. 2012 Aug;9(4):363-73. doi: 10.1586/epr.12.32.
Recombinant production of multiprotein complexes is an emerging focus in academic and pharmaceutical research and is expected to play a key role in addressing complex biological questions in health and disease. Here we describe MultiBac, a state-of-the-art eukaryotic expression technology utilizing an engineered baculovirus to infect insect cells. The robust and flexible concept of MultiBac allows for simultaneous expression of multiple proteins in a single cell, which can be used to produce protein complexes and to recapitulate metabolic pathways. The MultiBac system has been set up as an open-access platform technology at the European Molecular Biology Laboratory (EMBL) in Grenoble, France. The performance of this platform and its access modalities to the scientific community are detailed in this article. The MultiBac system has been instrumental for unlocking the function of a number of essential multiprotein complexes and recent examples are discussed. This article presents a novel concept for the customized production of glycosylated protein targets using SweetBac, a modified MultiBac vector system. Finally, this article outlines how MultiBac may further develop in the future to serve applications in both academic and industrial research and development.
多蛋白复合物的重组生产是学术和药物研究中的一个新兴焦点，预计将在解决健康和疾病领域复杂的生物学问题方面发挥关键作用。在这里，我们描述了 MultiBac，这是一种利用工程化杆状病毒感染昆虫细胞的最先进的真核表达技术。MultiBac 的强大而灵活的概念允许在单个细胞中同时表达多种蛋白质，可用于生产蛋白复合物和模拟代谢途径。MultiBac 系统已在法国格勒诺布尔的欧洲分子生物学实验室（EMBL）建立为开放访问的平台技术。本文详细介绍了该平台的性能及其向科学界提供的访问方式。该平台对于揭示许多必需的多蛋白复合物的功能起到了重要作用，本文讨论了最近的一些例子。本文提出了一种使用 SweetBac（一种经过改良的 MultiBac 载体系统）定制生产糖基化蛋白靶标的新概念。最后，本文概述了 MultiBac 未来如何进一步发展，以服务于学术和工业研发的应用。
