The School of Biochemistry and Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, Tankard's Close, Bristol, BS8 1TD, UK.
Geneva Biotech SARL, Avenue de la Roseraie 64, 1205, Genève, Switzerland.
BMC Biol. 2017 Oct 30;15(1):99. doi: 10.1186/s12915-017-0447-6.
The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.
MultiBac 杆状病毒/昆虫细胞表达载体系统被设计为一种用户友好的、模块化的工具包，用于生产用于结构生物学应用的多蛋白复合物。MultiBac 已经允许阐明许多分子机器的结构和功能，包括以前无法获得的高价值药物靶点。最近，MultiBac 的发展已经转向定制的杆状病毒基因组，这些基因组适用于各种应用，包括通过遗传密码扩展合成人工蛋白质。我们回顾了其中的一些发展，包括正在为哺乳动物应用重新设计 MultiBac 系统，特别是 CRISPR/Cas9 介导的基因编辑。
