Department of Chemistry, BITS, Pilani-K.K. Birla Goa Campus, NH 17 B Bypass Road, Zuarinagar, Goa 403726, India.
Talanta. 2012 Sep 15;99:610-5. doi: 10.1016/j.talanta.2012.06.045. Epub 2012 Jun 26.
A turn-on fluorescent probe for the detection of nitrite ion in water is developed based on diazotization reaction of the amino group of the probe in an acidic solution (pH 1). The probe responds selectively to nitrite ion over various other anions with a turn-on type fluorogenic change from colorless to orange by the formation of rhodamine B via an analyte triggered fragmentation process. The fluorescence titration is complete within 1h with 1 equivalent of nitrite ion. The probe is highly efficient, cost-effective and shows a detection limit of 4.6 ppb.
一种用于检测水中亚硝酸盐离子的荧光探针，基于探针在酸性溶液（pH 1）中氨基的重氮化反应。该探针对亚硝酸盐离子具有选择性响应，通过分析物触发的碎裂过程形成罗丹明 B，从而发生从无色到橙色的开环型荧光变化。在 1 小时内，1 当量的亚硝酸盐离子完成荧光滴定。该探针高效、经济实惠，检测限低至 4.6 ppb。
