Cai Meiyi, Chai Xiaoyun, Wang Xuedong, Wang Ting
College of Pharmacy, Weifang Medical University, Weifang, 261053, China.
Department of Organic Chemistry, College of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
J Fluoresc. 2017 Jul;27(4):1365-1371. doi: 10.1007/s10895-017-2071-9. Epub 2017 Mar 25.
The fluorescent detection of nitrite ions has been challenging owing to the poor acid-stability of typical fluorescent probes. Herein, an acid-inert rhodamine-based fluorescent probe has been developed for the fast detection of trace amount of nitrite ions with turn-on mode. The detection limit of nitrite ions was determined to be 9.4 nM (S/N = 3) and the linear range with high linear correlation was observed between 0.025 to 2.5 μM. A test paper method was developed for rapid visual detection of nitrite ions. Quantitative analysis of two real water samples also confirmed the reliability and sensitivity of the developed fluorescent method.
由于典型荧光探针的酸稳定性较差，亚硝酸盐离子的荧光检测一直具有挑战性。在此，已开发出一种基于罗丹明的酸惰性荧光探针，用于以开启模式快速检测痕量亚硝酸盐离子。亚硝酸盐离子的检测限确定为9.4 nM（信噪比=3），在0.025至2.5 μM之间观察到具有高线性相关性的线性范围。开发了一种试纸法用于快速目视检测亚硝酸盐离子。对两个实际水样的定量分析也证实了所开发荧光方法的可靠性和灵敏度。
