Meineri Giorgia, Cocolin Luca, Morelli Giada, Schievano Carlo, Atuahene David, Ferrocino Ilario
Department of Veterinary Sciences, School of Agriculture and Veterinary Medicine, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.
Vet Sci. 2023 Jul 3;10(7):434. doi: 10.3390/vetsci10070434.
Weaning entails numerous modifications of the intestinal structure and microbiota composition, making puppies at high risk of sickness during this delicate life stage. The aim of this study was to investigate the effects of a four-week administration of a supplement composed of ultramicronised Palmitoylethanolamide, bovine colostrum and (Normalia Extra, Innovet Italia Srl, Saccolongo, Italy) on markers of gut health and microbiome of weaning puppies. Twenty-nine four-week-old Golden Retriever puppies were randomly assigned to control (CG, = 13) and treated (TG, = 16) groups. During the whole experimental time, there were no differences between the groups with regard to average daily gain and faecal score. In TG, faecal calprotectin and zonulin values were statistically significantly decreased compared to CG, especially at week 8 (zonulin: 42.8 ± 1.54 ng/mL and 55.3 ± 42.8 ng/mL, and calprotectin: 2.91 ± 0.38 µg/g and 5.71 ± 0.43 µg/g, in TG and CG, respectively; < 0.0001 for both comparisons). Bacteria belonging to phylum decreased ( = 0.04), while those referring to genera and increased ( = 0.01 and = 0.04, respectively). The supplementation of the tested complementary feed can promote the intestinal health of puppies and therefore facilitate weaning by lowering gut inflammation.
断奶会导致肠道结构和微生物群组成发生诸多改变，使得幼犬在这个脆弱的生命阶段极易患病。本研究的目的是调查连续四周给予由超微粉化棕榈酰乙醇胺、牛初乳和（Normalia Extra，意大利Innovet Italia Srl公司，萨科隆戈，意大利）组成的补充剂对断奶幼犬肠道健康标志物和微生物组的影响。29只四周龄的金毛猎犬幼犬被随机分为对照组（CG，n = 13）和治疗组（TG，n = 16）。在整个实验期间，两组在平均日增重和粪便评分方面没有差异。在TG组中，与CG组相比，粪便钙卫蛋白和连蛋白值在统计学上显著降低，尤其是在第8周（连蛋白：TG组为42.8±1.54 ng/mL，CG组为55.3±42.8 ng/mL；钙卫蛋白：TG组为2.91±0.38 μg/g，CG组为5.71±0.43 μg/g；两项比较P均<0.0001）。属于门的细菌减少（P = 0.04），而属于属和属的细菌增加（分别为P = 0.01和P = 0.04）。补充受试补充饲料可促进幼犬的肠道健康，从而通过减轻肠道炎症来促进断奶。
