Robinson J Paul, Patsekin Valery, Holdman Cheryl, Ragheb Kathy, Sturgis Jennifer, Fatig Ray, Avramova Larisa V, Rajwa Bartek, Davisson V Jo, Lewis Nicole, Narayanan Padma, Li Nianyu, Qualls C W
Purdue University Cytometry Laboratories, Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA.
J Lab Autom. 2013 Feb;18(1):85-98. doi: 10.1177/2211068212456978. Epub 2012 Sep 10.
We have developed an automated system for drug screening using a single-cell-multiple functional response technology. The approach uses a semiautomated preparatory system, high-speed sample collection, and a unique analytical tool that provides instantaneous results for compound dilutions using 384-well plates. The combination of automation and rapid robotic sampling increases quality control and robustness. High-speed flow cytometry is used to collect single-cell results together with a newly defined analytical tool for extraction of IC(50) curves for multiple assays per cell. The principal advantage is the extreme speed of sample collection, with results from a 384-well plate being completed for both collection and data processing in less than 10 min. Using this approach, it is possible to extract detailed drug response information in a highly controlled fashion. The data are based on single-cell results, not populations. With simultaneous assays for different functions, it is possible to gain a more detailed understanding of each drug/compound interaction. Combined with integrated advanced data processing directly from raw data files, the process from sampling to analytical results is highly intuitive. Direct PubMed links allow review of drug structure and comparisons with similar compounds.
我们开发了一种使用单细胞多功能反应技术的药物筛选自动化系统。该方法采用半自动制备系统、高速样品采集以及一种独特的分析工具，该工具能为使用384孔板的化合物稀释提供即时结果。自动化与快速机器人采样相结合提高了质量控制和稳健性。高速流式细胞术用于收集单细胞结果，同时还有一种新定义的分析工具可用于提取每个细胞多种测定的IC(50)曲线。主要优点是样品采集速度极快，384孔板的采集和数据处理结果在不到10分钟内即可完成。使用这种方法，可以以高度可控的方式提取详细的药物反应信息。数据基于单细胞结果，而非群体结果。通过同时对不同功能进行测定，有可能更详细地了解每种药物/化合物的相互作用。结合直接从原始数据文件进行的集成高级数据处理，从采样到分析结果的过程非常直观。直接的PubMed链接允许查阅药物结构并与类似化合物进行比较。
