姜黄素存在下超声诱导鼻咽癌细胞发生线粒体自噬的透射电子显微镜观察
TEM observation of ultrasound-induced mitophagy in nasopharyngeal carcinoma cells in the presence of curcumin.
作者信息
Wang Xinna, Leung Albert Wingnang, Luo Jianmei, Xu Chuanshan
机构信息
Department of Ultrasound, The Affiliated Hospital, Xi'an Medical University, Xi'an;
出版信息
Exp Ther Med. 2012 Jan;3(1):146-148. doi: 10.3892/etm.2011.365. Epub 2011 Oct 13.
Abstract
The present study was designed to observe the initiation of mitophagy in tumor cells after ultrasound treatment in the presence of curcumin under transmission electron microscopy. Nasopharyngeal carcinoma CNE2 cells were incubated with 10 μM curcumin and then exposed to ultrasound for 8 sec at an intensity of 0.46 W/cm(2). Severely swollen mitochondria, disrupted mitochondria and mitophagy were noted in the CNE2 cells after ultrasound treatment in the presence of curcumin. Our findings demonstrated that ultrasound treatment in the presence of curcumin significantly initiated mitophagy in CNE2 cells, which suggests that mitophagy serves as an important event in the process of cell death of nasopharyngeal carcinoma cells.
摘要
本研究旨在通过透射电子显微镜观察姜黄素存在下超声处理后肿瘤细胞中自噬性线粒体的起始情况。将鼻咽癌CNE2细胞与10 μM姜黄素孵育，然后以0.46 W/cm(2)的强度暴露于超声8秒。在姜黄素存在下超声处理后的CNE2细胞中，观察到线粒体严重肿胀、线粒体破坏和自噬性线粒体。我们的研究结果表明，姜黄素存在下的超声处理显著启动了CNE2细胞中的自噬性线粒体，这表明自噬性线粒体是鼻咽癌细胞死亡过程中的一个重要事件。
相似文献
1
TEM observation of ultrasound-induced mitophagy in nasopharyngeal carcinoma cells in the presence of curcumin.姜黄素存在下超声诱导鼻咽癌细胞发生线粒体自噬的透射电子显微镜观察
Exp Ther Med. 2012 Jan;3(1):146-148. doi: 10.3892/etm.2011.365. Epub 2011 Oct 13.
2
Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells.低强度超声诱导鼻咽癌细胞的细胞破坏和自噬
Exp Ther Med. 2011 Sep;2(5):849-852. doi: 10.3892/etm.2011.317. Epub 2011 Jul 1.
3
Ultrasound-induced cell death of nasopharyngeal carcinoma cells in the presence of curcumin.超声刺激姜黄素诱导鼻咽癌细胞死亡。
Integr Cancer Ther. 2011 Mar;10(1):70-6. doi: 10.1177/1534735410377197. Epub 2010 Aug 11.
4
Mitochondrial damage in nasopharyngeal carcinoma cells induced by ultrasound radiation in the presence of hypocrellin B.
J Ultrasound Med. 2010 Jan;29(1):43-50. doi: 10.7863/jum.2010.29.1.43.
5
Hypocrellin B enhances ultrasound-induced cell death of nasopharyngeal carcinoma cells.竹红菌乙素增强超声诱导鼻咽癌细胞死亡。
Ultrasound Med Biol. 2010 Feb;36(2):336-42. doi: 10.1016/j.ultrasmedbio.2009.09.007. Epub 2009 Dec 16.
6
Ultrasound induces cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin.超声联合姜黄素诱导鼻咽癌细胞的细胞破坏。
Ultrasonics. 2011 Feb;51(2):165-70. doi: 10.1016/j.ultras.2010.07.006. Epub 2010 Aug 4.
7
Photodynamic effect of curcumin on NPC/CNE2 cells.姜黄素对鼻咽癌/CNE2细胞的光动力效应。
J Environ Pathol Toxicol Oncol. 2006;25(1-2):205-15. doi: 10.1615/jenvironpatholtoxicoloncol.v25.i1-2.120.
8
Curcumin exerts its antitumor activity through regulation of miR-7/Skp2/p21 in nasopharyngeal carcinoma cells.姜黄素通过调节鼻咽癌细胞中的miR-7/Skp2/p21发挥其抗肿瘤活性。
Onco Targets Ther. 2017 May 2;10:2377-2388. doi: 10.2147/OTT.S130055. eCollection 2017.
9
Apoptotic death in curcumin-treated NPC-TW 076 human nasopharyngeal carcinoma cells is mediated through the ROS, mitochondrial depolarization and caspase-3-dependent signaling responses.姜黄素处理的 NPC-TW 076 人鼻咽癌细胞中的细胞凋亡死亡是通过 ROS、线粒体去极化和 caspase-3 依赖性信号转导反应介导的。
Int J Oncol. 2011 Aug;39(2):319-28. doi: 10.3892/ijo.2011.1057. Epub 2011 May 26.
引用本文的文献
1
The role of mitochondrial dysfunction in the pathogenesis of Alzheimer's disease and future strategies for targeted therapy.线粒体功能障碍在阿尔茨海默病发病机制中的作用及靶向治疗的未来策略。
Eur J Med Res. 2025 May 31;30(1):434. doi: 10.1186/s40001-025-02699-w.
2
Curcumin liposomes alleviate senescence of bone marrow mesenchymal stem cells by activating mitophagy.姜黄素脂质体通过激活线粒体自噬减轻骨髓间充质干细胞的衰老。
Sci Rep. 2024 Dec 28;14(1):31291. doi: 10.1038/s41598-024-82614-1.
3
Mitophagy: A novel perspective for insighting into cancer and cancer treatment.自噬：深入洞察癌症和癌症治疗的新视角。
Cell Prolif. 2022 Dec;55(12):e13327. doi: 10.1111/cpr.13327. Epub 2022 Oct 5.
4
Mitophagy and mitochondrial dynamics in type 2 diabetes mellitus treatment.2 型糖尿病治疗中的自噬和线粒体动力学。
Aging (Albany NY). 2022 Mar 24;14(6):2902-2919. doi: 10.18632/aging.203969.
5
The prohibitin-binding compound fluorizoline inhibits mitophagy in cancer cells.与抑制素结合的化合物氟代唑啉可抑制癌细胞中的线粒体自噬。
Oncogenesis. 2021 Sep 27;10(9):64. doi: 10.1038/s41389-021-00352-9.
6
Recent progress in sono-photodynamic cancer therapy: From developed new sensitizers to nanotechnology-based efficacy-enhancing strategies.声动力癌症治疗的最新进展：从新型敏化剂的研发到基于纳米技术的疗效增强策略
Acta Pharm Sin B. 2021 Aug;11(8):2197-2219. doi: 10.1016/j.apsb.2020.12.016. Epub 2020 Dec 21.
7
MAPK1/3 kinase-dependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis.MAPK1/3 激酶依赖性 ULK1 降解减弱了线粒体自噬，促进了乳腺癌骨转移。
Autophagy. 2021 Oct;17(10):3011-3029. doi: 10.1080/15548627.2020.1850609. Epub 2020 Dec 7.
8
Curcumin, a Multifaceted Hormetic Agent, Mediates an Intricate Crosstalk between Mitochondrial Turnover, Autophagy, and Apoptosis.姜黄素，一种多面性的应激适应原，介导线粒体更新、自噬和凋亡之间复杂的相互作用。
Oxid Med Cell Longev. 2020 Jul 18;2020:3656419. doi: 10.1155/2020/3656419. eCollection 2020.
9
Effects of Plant and Animal Natural Products on Mitophagy.植物和动物天然产物对自噬的影响。
Oxid Med Cell Longev. 2020 Mar 10;2020:6969402. doi: 10.1155/2020/6969402. eCollection 2020.
10
Preventive Effect of Curcumin Against Chemotherapy-Induced Side-Effects.姜黄素对化疗引起的副作用的预防作用。
Front Pharmacol. 2018 Nov 27;9:1374. doi: 10.3389/fphar.2018.01374. eCollection 2018.
本文引用的文献
1
Ultrasound induces cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin.超声联合姜黄素诱导鼻咽癌细胞的细胞破坏。
Ultrasonics. 2011 Feb;51(2):165-70. doi: 10.1016/j.ultras.2010.07.006. Epub 2010 Aug 4.
2
Ultrasound-induced cell death of nasopharyngeal carcinoma cells in the presence of curcumin.超声刺激姜黄素诱导鼻咽癌细胞死亡。
Integr Cancer Ther. 2011 Mar;10(1):70-6. doi: 10.1177/1534735410377197. Epub 2010 Aug 11.
3
5-Aminolaevulinic acid enhances ultrasound-induced mitochondrial damage in K562 cells.5-氨基酮戊酸增强超声诱导的 K562 细胞线粒体损伤。
Ultrasonics. 2010 Aug;50(8):777-81. doi: 10.1016/j.ultras.2010.03.004. Epub 2010 Mar 21.
4
High-intensity focused ultrasound in the management of prostate cancer.高强度聚焦超声在前列腺癌治疗中的应用。
Expert Rev Med Devices. 2010 Mar;7(2):209-17. doi: 10.1586/erd.09.66.
5
Mitochondrial damage in nasopharyngeal carcinoma cells induced by ultrasound radiation in the presence of hypocrellin B.
J Ultrasound Med. 2010 Jan;29(1):43-50. doi: 10.7863/jum.2010.29.1.43.
6
Hypocrellin B enhances ultrasound-induced cell death of nasopharyngeal carcinoma cells.竹红菌乙素增强超声诱导鼻咽癌细胞死亡。
Ultrasound Med Biol. 2010 Feb;36(2):336-42. doi: 10.1016/j.ultrasmedbio.2009.09.007. Epub 2009 Dec 16.
7
Assessment of high intensity focused ultrasound for the treatment of prostate cancer.
Acta Chir Belg. 2009 Oct;109(5):581-6. doi: 10.1080/00015458.2009.11680492.
8
Hematoporphyrin monomethyl ether enhances the killing action of ultrasound on osteosarcoma in vivo.血卟啉单甲醚增强超声对骨肉瘤的体内杀伤作用。
J Ultrasound Med. 2009 Dec;28(12):1695-702. doi: 10.7863/jum.2009.28.12.1695.
9
Inhibition of autophagy induced by proteasome inhibition increases cell death in human SHG-44 glioma cells.蛋白酶体抑制诱导的自噬抑制会增加人SHG-44胶质瘤细胞的细胞死亡。
Acta Pharmacol Sin. 2009 Jul;30(7):1046-52. doi: 10.1038/aps.2009.71.
10
Apoptosis, autophagy and cell cycle arrest following photodamage to mitochondrial interior.线粒体内部光损伤后的细胞凋亡、自噬和细胞周期阻滞
Apoptosis. 2009 Mar;14(3):276-86. doi: 10.1007/s10495-008-0292-8.
Zotero 插件