Wang Xinna, Leung Albert Wingnang, Luo Jianmei, Xu Chuanshan
Department of Ultrasound, The Affiliated Hospital, Xi'an Medical University, Xi'an;
Exp Ther Med. 2012 Jan;3(1):146-148. doi: 10.3892/etm.2011.365. Epub 2011 Oct 13.
The present study was designed to observe the initiation of mitophagy in tumor cells after ultrasound treatment in the presence of curcumin under transmission electron microscopy. Nasopharyngeal carcinoma CNE2 cells were incubated with 10 μM curcumin and then exposed to ultrasound for 8 sec at an intensity of 0.46 W/cm(2). Severely swollen mitochondria, disrupted mitochondria and mitophagy were noted in the CNE2 cells after ultrasound treatment in the presence of curcumin. Our findings demonstrated that ultrasound treatment in the presence of curcumin significantly initiated mitophagy in CNE2 cells, which suggests that mitophagy serves as an important event in the process of cell death of nasopharyngeal carcinoma cells.
本研究旨在通过透射电子显微镜观察姜黄素存在下超声处理后肿瘤细胞中自噬性线粒体的起始情况。将鼻咽癌CNE2细胞与10 μM姜黄素孵育，然后以0.46 W/cm(2)的强度暴露于超声8秒。在姜黄素存在下超声处理后的CNE2细胞中，观察到线粒体严重肿胀、线粒体破坏和自噬性线粒体。我们的研究结果表明，姜黄素存在下的超声处理显著启动了CNE2细胞中的自噬性线粒体，这表明自噬性线粒体是鼻咽癌细胞死亡过程中的一个重要事件。
