低氧和正常条件下培养的大鼠骨膜细胞的成骨差异
Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions.
作者信息
Ichijima Takehiro, Matsuzaka Kenichi, Tonogi Morio, Yamane Gen-Yuki, Inoue Takashi
机构信息
Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College, Ichikawa General Hospital, Ichikawa-shi, Chiba 272-8513;
出版信息
Exp Ther Med. 2012 Feb;3(2):165-170. doi: 10.3892/etm.2011.393. Epub 2011 Nov 28.
The aim of the present study was to investigate the osteogenic capability of rat calvarial periosteal cells in hypoxic conditions in vitro. Periosteum was obtained from the calvarial bone of Sprague-Dawley rats. Following primary tissue culture, subcultured cells were used in hypoxic or normal conditions. On days 1, 2, 3 and 4 following the cell culture, cell proliferation and mRNA and protein expression levels were evaluated. No significant difference in the cell proliferation rate was found between the normal and hypoxic condition groups. The hypoxic condition group exhibited a stronger expression of hypoxia-inducible factor (HIF)1α, vascular endothelial growth factor (VEGF), Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN) and periostin at the mRNA level compared to that of the normal condition group. The hypoxic condition group also exhibited a stronger expression of HIF1α, VEGF, bone morphogenetic protein (BMP)2, Runx2, ALP and BSP at the protein level compared to that of the normal condition group. In conclusion, periosteal cells cultured in hypoxic conditions demonstrated activated osteogenic capability in vitro.
本研究的目的是在体外低氧条件下研究大鼠颅骨骨膜细胞的成骨能力。骨膜取自Sprague-Dawley大鼠的颅骨。原代组织培养后，将传代培养的细胞用于低氧或正常条件下。在细胞培养后的第1、2、3和4天，评估细胞增殖以及mRNA和蛋白质表达水平。正常条件组和低氧条件组之间的细胞增殖率未发现显著差异。与正常条件组相比，低氧条件组在mRNA水平上缺氧诱导因子（HIF）1α、血管内皮生长因子（VEGF）、Runx2、碱性磷酸酶（ALP）、骨唾液蛋白（BSP）、骨钙素（OCN）和骨膜蛋白的表达更强。与正常条件组相比，低氧条件组在蛋白质水平上HIF1α、VEGF、骨形态发生蛋白（BMP）2、Runx2、ALP和BSP的表达也更强。总之，在低氧条件下培养的骨膜细胞在体外表现出激活的成骨能力。
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