Wu DI, Guo Ze, Min Wei, Zhou Bingrong, Li Mingna, Li Wei, Luo Dan
Departments of Dermatology, and.
Exp Ther Med. 2012 Mar;3(3):437-442. doi: 10.3892/etm.2011.439. Epub 2011 Dec 28.
The translationally controlled tumor protein (TCTP) is an anti-apoptotic protein, which is highly expressed in several human cancer types. However, the role of TCTP in skin cancers, including squamous cell carcinoma (SCC), has not been investigated. In this study, we analyzed the expression of TCTP in cutaneous SCC samples using immunohistochemistry in two epidermoid SCC cell lines, A431 and SCL-1, using western blot analysis. We further investigated the role of TCTP in skin cancinogenesis by silencing the TPT1 gene using small interfering RNA (siRNA) in the SCC cell line A431. Our results demonstrated that TCTP was overexpressed in cutaneous SCC cells, compared to normal skin keratinocytes. In addition, the expression of TCTP in skin SCC significantly increased with the grade of malignancy. Western blot analysis further confirmed that the expression of TCTP in the cell lines, A431 and SCL-1, was significantly higher compared to that in the normal keratinocyte cell line, HaCaT. The expression of TCTP in A431 cells was significantly downregulated by transfection with our specifically designed TCTP siRNA. We found that downregulation of TCTP expression was associated with decreased cell proliferation and increased apoptosis in A431 cells. These results suggest that the TPT1 gene may be a potential therapeutic target in skin SCC through a siRNA approach.
翻译调控肿瘤蛋白（TCTP）是一种抗凋亡蛋白，在多种人类癌症类型中高表达。然而，TCTP在包括鳞状细胞癌（SCC）在内的皮肤癌中的作用尚未得到研究。在本研究中，我们使用免疫组织化学分析了皮肤SCC样本中TCTP的表达，并在两种表皮样SCC细胞系A431和SCL-1中使用蛋白质印迹分析。我们通过在SCC细胞系A431中使用小干扰RNA（siRNA）沉默TPT1基因，进一步研究了TCTP在皮肤癌发生中的作用。我们的结果表明，与正常皮肤角质形成细胞相比，TCTP在皮肤SCC细胞中过表达。此外，TCTP在皮肤SCC中的表达随着恶性程度的增加而显著增加。蛋白质印迹分析进一步证实，与正常角质形成细胞系HaCaT相比，细胞系A431和SCL-1中TCTP的表达显著更高。通过转染我们专门设计的TCTP siRNA，A431细胞中TCTP的表达显著下调。我们发现TCTP表达的下调与A431细胞中细胞增殖的减少和凋亡的增加有关。这些结果表明，通过siRNA方法，TPT1基因可能是皮肤SCC的一个潜在治疗靶点。
