Subramanian Elaiya Raja, Gopi Daisy Nino, Sudalaimani Dinesh Kumar, Ramamoorthy Kalidas, Balakrishnan Subburathinam, Selvan Christyraj Jackson Durairaj, Arumugaswami Vaithilingaraja, Sivasubramaniam Sudhakar
Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
Viral Hepatitis and Gene/Cell Therapy Laboratory, CSMC Viral Vector Core, University of California, Los Angeles, United States of America.
PLoS One. 2017 Apr 12;12(4):e0175319. doi: 10.1371/journal.pone.0175319. eCollection 2017.
TCTP (Translationally Controlled Tumour Protein) is a multifunctional protein that plays a role in the development, immune system, tumour reversion, and maintenance of stem cells. The mRNA of the Tpt1 gene is over-expressed during liver regeneration. But, the function of the protein in regeneration is not known. To study the role of the protein in regeneration, the earthworm Eudrilus eugeniae was chosen. First, the full length cDNA of the Tpt1 gene was sequenced. The size of the cDNA is 504 bp and the protein has 167 amino acids. The highest level of TCTP expression was documented in the worm after three days of regeneration. The protein was found to be expressed specifically in the epithelial layer of the skin. During regeneration, the protein expression was found to be the highest at the tip of blastema. The pharmacological suppression of TCTP using nutlin-3 and TCTP RNAi experiments resulted in the failure of the regeneration process. The suppression of TCTP caused the arrest of proliferation in posterior amputated worms. The severe cell death was documented in the amputated region of nutlin-3 injected worm. The silencing of TCTP has blocked the modification of clitellar segments. The experiments confirm that TCTP has major functions in the upstream signalling of cell proliferation in the early regeneration process in E. eugeniae.
翻译控制肿瘤蛋白（TCTP）是一种多功能蛋白，在发育、免疫系统、肿瘤逆转和干细胞维持中发挥作用。Tpt1基因的mRNA在肝脏再生过程中过度表达。但是，该蛋白在再生中的功能尚不清楚。为了研究该蛋白在再生中的作用，选用了蚯蚓真蚓。首先，对Tpt1基因的全长cDNA进行了测序。该cDNA大小为504 bp，该蛋白有167个氨基酸。再生三天后，在蚯蚓中记录到TCTP的最高表达水平。发现该蛋白在皮肤上皮层特异性表达。在再生过程中，发现该蛋白在芽基顶端的表达最高。使用nutlin-3对TCTP进行药理学抑制和TCTP RNA干扰实验导致再生过程失败。TCTP的抑制导致后段切断的蚯蚓增殖停滞。在注射nutlin-3的蚯蚓的切断区域记录到严重的细胞死亡。TCTP的沉默阻止了环带节段的修饰。实验证实，TCTP在真蚓早期再生过程中细胞增殖的上游信号传导中具有主要功能。
