Yu Jun, Huang Xin, Wu Qirong, Wang Jun, Yu Xiaoyan, Zhao Peiquan
Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine;
Exp Ther Med. 2012 May;3(5):803-806. doi: 10.3892/etm.2012.486. Epub 2012 Feb 13.
The purpose of the present study was to investigate the effect of the A(2A) receptor antagonist (SCH 442416) on the mRNA expression of glutamate aspartate transporter (GLAST) and glutamine synthetase (GS) in rat retinal Müller cells under hypoxic conditions in vitro. Immunofluorescent staining of GS and GFAP was used for the identification of Müller cells. The GLAST and GS mRNA expression of Müller cells treated with 0.1, 1 and 10 μM SCH 442416 under hypoxic conditions was examined by real-time PCR. Müller cells increased the mRNA expression of GLAST under hypoxic conditions; those treated with 0.1 μM SCH 442416 showed a further significant increase in the mRNA expression of GLAST in vitro. Although the mRNA expression of GS was decreased under hypoxic conditions, the mRNA expression was increased when Müller cells were treated with 0.1 μM SCH 442416. A(2A) receptor antagonist increased the GLAST and GS expression of Müller cells and accelerated the clearance of extracellular glutamate under hypoxic conditions in vitro.
本研究的目的是在体外低氧条件下，研究A(2A)受体拮抗剂（SCH 442416）对大鼠视网膜Müller细胞中谷氨酸天冬氨酸转运体（GLAST）和谷氨酰胺合成酶（GS）mRNA表达的影响。采用GS和GFAP免疫荧光染色鉴定Müller细胞。通过实时PCR检测在低氧条件下用0.1、1和10 μM SCH 442416处理的Müller细胞的GLAST和GS mRNA表达。Müller细胞在低氧条件下GLAST的mRNA表达增加；在体外，用0.1 μM SCH 442416处理的细胞GLAST的mRNA表达进一步显著增加。虽然在低氧条件下GS的mRNA表达降低，但当Müller细胞用0.1 μM SCH 442416处理时，mRNA表达增加。A(2A)受体拮抗剂在体外低氧条件下增加了Müller细胞的GLAST和GS表达，并加速了细胞外谷氨酸的清除。
