DU Yuan, Wang Tian, Jiang Na, Ren Ru-Tong, Li Chong, Li Chang-Kun, Fu Feng-Hua
Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
Exp Ther Med. 2012 May;3(5):818-822. doi: 10.3892/etm.2012.479. Epub 2012 Feb 13.
Methyl parathion, a highly cytotoxic insecticide, has been used in agricultural pest control for several years. The present study investigated the protective effect of sodium aescinate (SA, the sodium salt of aescin) against liver injury induced by methyl parathion. Forty male Sprague-Dawley rats were randomly divided into 5 groups of 8 animals: the control group; the methyl parathion (15 mg/kg) poisoning (MP) group; and the MP plus SA at doses of 0.45, 0.9 and 1.8 mg/kg groups. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and acetylcholinesterase (AChE) in the plasma were assayed. Nitric oxide (NO) and antioxidative parameters were measured. Histopathological examination of the liver was also performed. The results revealed that SA had no effect on AChE. Treatment with SA decreased the activities of ALT and AST, and the levels of malondialdehyde and NO. Treatment with SA also increased the level of glutathione and the activities of superoxide dismutase and glutathione peroxidase. SA administration also ameliorated liver injury induced by methyl parathion poisoning. The findings indicate that SA protects against liver injury induced by methyl parathion and that the mechanism of action is related to the antioxidative and anti-inflammatory effects of SA.
甲基对硫磷是一种具有高度细胞毒性的杀虫剂，已用于农业害虫防治数年。本研究调查了七叶皂苷钠（SA，七叶皂苷的钠盐）对甲基对硫磷诱导的肝损伤的保护作用。将40只雄性Sprague-Dawley大鼠随机分为5组，每组8只动物：对照组；甲基对硫磷（15 mg/kg）中毒（MP）组；以及MP加0.45、0.9和1.8 mg/kg剂量SA的组。测定血浆中的丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乙酰胆碱酯酶（AChE）。测量一氧化氮（NO）和抗氧化参数。还对肝脏进行了组织病理学检查。结果显示SA对AChE无影响。SA治疗降低了ALT和AST的活性，以及丙二醛和NO的水平。SA治疗还提高了谷胱甘肽水平以及超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。SA给药也改善了甲基对硫磷中毒诱导的肝损伤。这些发现表明SA可预防甲基对硫磷诱导的肝损伤，其作用机制与SA的抗氧化和抗炎作用有关。
