Immer Ulrike, Haas-Lauterbach Sigrid
R-Biopharm AG, An der neuen Bergstrasse 17, 64297 Darmstadt, Germany.
J AOAC Int. 2012 Jul-Aug;95(4):1118-24. doi: 10.5740/jaoacint.cs2012_01.
The Working Group on Prolamin Analysis and Toxicity (WGPAT) organized a collaborative study to confirm whether the two R5 antibody-based ELISA test kits are able to detect gliadin in the lower mg/kg (ppm) level. Twenty laboratories investigated 12 blind-coded samples, spiked and naturally contaminated, to show the possibility of determining traces of gliadin in heat-treated or nonheat-treated foods by ELISA. It was shown that very small amounts of gliadin (below 100 ppm) could be detected by ELISA with a reproducibility RSD(R) (37%) and a repeatability RSD, (27%) common for ELISA under these conditions. The recovery of gliadin from the spiked samples was between 84 and 109%, based on the results of all laboratories, including those with poor performance. No false positives were found by the method (P < or =0.05), but one negative sample was contaminated during the bakery process. It is recommended that the method be accepted by AOAC as Official First Action.
醇溶蛋白分析与毒性工作组（WGPAT）组织了一项合作研究，以确认两种基于R5抗体的酶联免疫吸附测定（ELISA）试剂盒是否能够检测出毫克/千克（ppm）级别的醇溶蛋白。20个实验室对12个经过盲编码的样品进行了研究，这些样品包括添加了醇溶蛋白的和天然受污染的，以展示通过ELISA法测定热处理或未热处理食品中痕量醇溶蛋白的可能性。结果表明，在此条件下，ELISA法能够检测出极少量的醇溶蛋白（低于100 ppm），其重现性相对标准偏差RSD(R)为37%，重复性相对标准偏差RSD为27%，这在ELISA法中是常见的。基于所有实验室的结果，包括那些表现不佳的实验室，添加了醇溶蛋白的样品的回收率在84%至109%之间。该方法未发现假阳性结果（P≤0.05），但有一个阴性样品在烘焙过程中受到了污染。建议美国官方分析化学师协会（AOAC）将该方法作为“官方首次行动”予以接受。
